枸杞子加丹参对RCS(rdy-/-,p-/-)大鼠视网膜匀浆中cAMP、cGMP含量的影响  

Effects of Fructus Lycii and Radix Salviae Miltiorrhizae on Contents of cAMP and cGMP in Retinal Homogenate of RCS(rdy-/-, p-/-) Rats

作　　者：徐剑 周亚莎 彭俊[3] 潘坤[1] 吴权龙[3] 李建超[1] 刘悦[1] 王英[1] 彭清华[1] XU Jian;ZHOU Yasha;PENG Jun;PAN Kun;WU Quanlong;LI Jianchao;LIU Yue;WANG Ying;PENG Qinghua(Hunan University of Chinese Medicine,Changsha,Hunan 410208,China;Center of Ophthalmology,Oriental Hospital Affiliated to Tongji University,Shanghai 200120,China;The First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha,Hunan 410007,China)

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]上海市同济大学附属东方医院眼科中心,上海200120 [3]湖南中医药大学第一附属医院,湖南长沙410007

出　　处：《湖南中医药大学学报》2020年第7期784-787,共4页Journal of Hunan University of Chinese Medicine

基　　金：国家自然科学基金资助项目(81804150);湖南省自然科学基金资助项目(2019JJ40226);湖南中医药大学中医诊断学国家重点学科开放基金优先资助项目(2015ZYZD02);湖南省教育厅创新平台开放基金项目(16K065);湖南省高层次卫生人才“225”工程培养项目资助;中医药防治五官科疾病湖南省重点实验室建设项目(2017TP1018);长沙市科技计划项目(kc1704005);中央财政支持地方高校建设项目;国家中医药管理局中医眼科学重点学科建设项目;湖南省中医五官科学重点学科建设项目;湖南中医药大学中医学国内一流建设学科。

摘　　要：目的探讨枸杞子加丹参对视网膜色素变性模型大鼠RCS(rdy-/-, p-/-)视网膜匀浆中cAMP含量、cGMP含量以及cAMP/cGMP值的影响。方法实验动物随机分为5组:空白组为RCS(rdy+/+, p+/+)大鼠8只,模型组、枸杞子组、丹参组及枸杞子加丹参组(杞参组)为RCS(rdy-/-, p-/-)大鼠各8只,每组均雌雄各半。5组大鼠分别给予生理盐水、生理盐水、枸杞子、丹参、枸杞子加丹参灌胃。灌胃28 d后,ELISA法测定视网膜组织匀浆中cAMP、cGMP含量及cAMP/cGMP值。结果与空白组比较,模型组大鼠视网膜匀浆组织中cAMP、cGMP含量及cAMP/cGMP值均增高,差异有显著统计学意义(P<0.01);与模型组比较,杞参组视网膜匀浆组织中cAMP、cGMP含量及cAMP/cGMP值均明显降低,差异有显著统计学意义(P<0.01)。结论 "补虚活血"代表中药枸杞子加丹参可以降低RCS(rdy-/-,p-/-)大鼠视网膜组织匀浆中cAMP、cGMP含量及cAMP/cGMP值,改善其"虚"证指标。Objective To study the effects of Fructus Lycii and Radix Salviae Miltiorrhizae on the content of cAMP, cGMP and cAMP/cGMP retinal homogenate of RCS(rdy-/-,p-/-) rats.Methods The experimental animals were randomly divided into5 group.The blank group had 8 RCS(rdy+/+,p+/+) rats.Each of the Fructus Lycii group, the Radix Salviae Miltiorrhizae group, the Fructus Lycii and Radix Salviae Miltiorrhizae(Qishen) group had 8 RCS(rdy-/-, p-/-) rats.Each group had half male and half female rats.The 5 groups were respectively filled the stomach with normal saline, normal saline, Fructus Lycii, Radix Salviae Miltiorrhizae, Fructus Lycii and Radix Salviae Miltiorrhizae by gavage.After 28 days’ gavage, the content of cAMP, cGMP and cAMP/cGMP in retinal homogenate were determined by ELISA.Results Compared with the blank group, the content of cAMP,cGMP and cAMP/cGMP in the retinal homogenate tissue of the model group were increased, and the difference was statistically significant(P <0.01);Compared with the model group, the content of cAMP and cGMP and the value of cAMP/cGMP in the retinal homogenate tissue of Qishen group were significantly reduced, and the difference was statistically significant(P <0.01).Conclusion The representative Chinese materia medica for "tonifying deficiency and activating blood circulation" Fructus Lycii and Radix Salviae Miltiorrhizae can reduce the contents of cAMP, cGMP and cAMP/cGMP in the retinal homogenate tissue of RCS(rdy-/-,p-/-) rats and improve the index of "deficiency".

关 键 词：视网膜色素变性 枸杞子 丹参 RCS大鼠 CAMP CGMP 

分 类 号：R276.7[医药卫生—中医五官科学]