野生建兰根际与根内共生细菌种群结构差异分析  

Microbial Communities in Rhizosphere and Root-Endosphere of Wild Cymbidium ensifolium

作　　者：谢泰祥 张清华[2] 周杰 陈娟 马山虎 艾叶 XIE Taixiang;ZHANG Qinghua;ZHOU Jie;CHEN Juan;MA Shanhu;AI Ye(College of Landscape Architecture,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;College of Forestry,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)

机构地区：[1]福建农林大学园林学院,福建福州350002 [2]福建农林大学林学院,福建福州350002

出　　处：《福建农业学报》2020年第5期560-568,共9页Fujian Journal of Agricultural Sciences

基　　金：福建省自然科学基金项目(2016J01703);国家自然科学基金项目(31700618);福建省种业创新与产业化工程项目(ZYCX-LY-2017005)。

摘　　要：【目的】比较野生建兰根际和根内细菌的种群结构差异,为研究根共生细菌与建兰的营养关系奠定理论基础。【方法】采用高通量测序技术鉴定福州鼓山野生建兰根际和根内共生细菌,分析其种类多样性和种群结构。【结果】建兰根际、根内拥有种类多样的细菌,辛普森指数分别为0.99、0.95;香农指数比较显示建兰根际细菌种类多样性显著高于根内。建兰根际拥有10门细菌,优势门为变形菌门(60.5%)、酸杆菌门(20.5%)、放线菌门(15.3%),根内优势细菌门分别为变形菌门(75.3%)、酸杆菌门(7.3%)、放线菌门(14.6%)。根际与根内丰度最高的10个细菌属中,Solibacter、Acidipila仅在根际中,戴氏菌、新鞘氨醇菌和Granulicella仅存在于根内。根际中酸杆菌门酸杆菌纲的丰度显著高于根内,11个差异显著的目,仅肠杆菌目在根内的丰度大于根际,根际有12个属的丰度显著高于根内。【结论】建兰根际与根内细菌种类丰富度存在显著性差异,其原因可能是不同生态位的细菌对建兰的生物学功能存在较大差异。【Objective】Community diversity and structures of the bacteria in the rhizosphere and endosphere of wild Cymbidium ensifolium were studied to understand the symbiotic relationship between them.【Method】The high-throughput sequencing method was applied to identify the bacteria in the rhizosphere soil and the root-endosphere of C.ensifolium at locations in Mt.Gushan,Fuzhou where the wild orchids were found.Species diversity and community structures of the spheres were compared.【Result】Numerous species of bacteria were found in the specimens.The Simpson index in the rhizosphere soil was determined to be of 0.99,and that in the root-endosphere 0.95.The Shannon indices indicated that the rhizosphere was significantly more diverse on species than the root-endosphere.There were 10 phyla of bacteria in the rhizosphere with the dominant ones being Proteobacteria(60.5%),Acidobacteria(20.5%),and Actinobacteria(15.3%).In the root-endosphere,the prevailing phyla were Proteobacteria(75.3%),Acidobacteria(7.3%),and Actinobacteria(14.6%).Among the 10 bacterial genera in the highest abundance,Solibacter and Acidipila existed only in the rhizosphere,while Dyella,Novosphingobium,and Granulicella only in the root-endosphere.The abundance of Acidobacteria Acidobacteriia bacteria in the rhizosphere were significantly higher than those in the root-endosphere.Of the 11 significantly different orders,only Enterobacteriales showed a greater abundance in the root-endosphere than in the rhizosphere.There were 12 genera significantly more abundant in the rhizosphere than in the root-endosphere.【Conclusion】The significantly different microbial community diversity and structures in the field rhizosphere and root-endosphere of wild C.ensifolium grown at the same area might reflect a highly complex synergism between the environment and the plants.

关 键 词：建兰 根际细菌 根内生细菌 种群结构 种类多样性 

分 类 号：S93[农业科学—渔业资源] S682.31[农业科学—水产科学]