硒半胱氨酸插入序列结合蛋白2基因rs3211703多态性与缺血性中风血瘀证及气虚证显著关联  

SECISBP2 Gene rs3211703 Polymorphism is Significantly Associated with Ischemic Stroke and Blood Stasis Syndrome and Qi Deficiency Syndrome

作　　者：古联[1,2] 陈卓 严雁[1] 龙建雄[3] 朱路路 苏莉[3] GU Lian;CHEN Zhuo;YAN Yan;LONG Jianxiong;ZHU Lulu;SU Li(The First Affiliated Hospital of the Guangxi University of Chinese Medicine,Nanning 530023,Guangxi,China;Guangxi University of Chinese Medicine,Nanning 530001,Guangxi,China;School of Public Health,Guangxi Medical University,Nanning 530001,Guangxi,China)

机构地区：[1]广西中医药大学第一附属医院,广西南宁530023 [2]广西中医药大学,广西南宁530001 [3]广西医科大学公共卫生学院,广西南宁530021

出　　处：《中华中医药学刊》2020年第6期23-27,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金(81573756,81660741)。

摘　　要：目的探讨硒半胱氨酸插入序列结合蛋白2(SECISBP2,SBP2)基因rs3211703多态性与缺血性中风(IS)中医证型的关联性。方法共纳入汉族缺血性中风患者774例,正常对照793例。采用《中风病辨证诊断标准》对IS患者进行中医辨证。运用MassARRAY SNP基因分型技术进行基因分型检测。采用PLINK软件进行遗传关联分析。结果SECISBP2基因rs3211703多态性与缺血性中风血瘀证[加性模型:OR(95%CI)=1.36(1.06~1.74),P=0.015;显性模型:OR(95%CI)=1.45(1.04~2.01),P=0.028;等位模型:OR(95%CI)=1.38(1.07~1.77),P=0.013]、气虚证发生风险均显著关联[显性模型:OR(95%CI)=1.72(1.10~2.68),P=0.018;等位模型:OR(95%CI)=1.40(1.00~1.95),P=0.048];校正年龄、性别后,SECISBP2基因rs3211703多态性与缺血性中风血瘀证[加性模型:ORadj(95%CI)=1.36(1.06~1.74),Padj=0.014;显性模型:ORadj(95%CI)=1.45(1.04~2.02),Padj=0.027]、气虚证[显性模型:ORadj(95%CI)=1.69(1.08~2.65),Padj=0.021]发生风险的关联仍具有统计学意义。结论SECISBP2基因rs3211703多态性可能影响中国汉族人缺血性中风血瘀证及气虚证的发生。Objective To investigate the correlation between the polymorphism of the secysteine insertion sequence binding protein 2(SECISBP2,SBP2)gene rs3211703 and the TCM syndromes of ischemic stroke(IS).Methods Totally 774 patients with ischemic stroke and 793 normal controls were included.The syndrome differentiation diagnosis standard of apoplexy was adopted to carry out TCM syndrome differentiation for IS patients.MassARRAY SNP genotyping technique was used for genotyping detection.PLINK software was used for genetic association analysis.Results SECISBP2 gene rs3211703 polymorphism and ischemic stroke blood stasis syndrome was significantly related[additive model:OR(95%CI)=1.36(1.06~1.74),P=0.015;Dominant model:OR(95%CI)=1.45(1.04~2.01),P=0.028;Allele model:OR(95%CI)=1.38(1.07~1.77),P=0.013].The risk of Qi deficiency syndrome was significantly correlated[dominant model:OR(95%CI)=1.72(1.10~2.68),P=0.018;Allelic model:OR(95%CI)=1.40(1.00~1.95),P=0.048].After adjusting age and gender,SECISBP2 gene rs3211703 polymorphism and ischemic stroke blood stasis syndrome[additive model:ORadj(95%CI)=1.36(1.06~1.74),Padj=0.014;Dominant model:ORadj(95%CI)=1.45(1.04~2.02),Padj=0.027]and deficiency of Qi syndrome[dominant model:ORadj(95%CI)=1.69(1.08~2.65),Padj=0.021]were still statistically significant.Conclusion The polymorphism of SECISBP2 gene rs3211703 may affect the occurrence of ischemic stroke blood stasis syndrome and Qi deficiency syndrome in Han Chinese.

关 键 词：缺血性中风 硒半胱氨酸插入序列结合蛋白2 单核苷酸多态性 血瘀证 气虚证 证候 同病异证 

分 类 号：R255.2[医药卫生—中医内科学]