2种中药提取液对兔球虫卵囊孢子化的影响  

Study on the Effect of Two Chinese Medicine Extracts on the Cystosporidization of Rabbit Coccidioides

作　　者：徐瑞欣 吕虹 应慧惠 何瑶 吴茜 廖娟[1] XU Rui-xin;LV Hong;YING Hui-hui;HE Yao;WU Qian;LIAO Juan(Engineering Research Center of Sichuan Province Higher School of Local Chicken Breeds Industrialization in Southern Sichuan,Leshan Normal University,Leshan 614000,China)

机构地区：[1]乐山师范学院,川南地方品种鸡产业化四川省高等学校工程研究中心,四川乐山614000

出　　处：《当代畜牧》2020年第4期38-43,共6页Contemporary Animal Husbandry

摘　　要：为了研究青蒿、秦皮这2种中草药不同质量浓度溶液对兔球虫卵囊孢子化的影响,笔者采用饱和食盐水漂浮法分离兔球虫卵囊,再将未孢子化的兔球虫卵囊分别置于不同质量浓度(0.25 g/m L、0.50 g/m L、1.00 g/m L)的青蒿及秦皮中药提取液中,28℃恒温连续培养5 d。每24 h在显微镜下观察兔球虫卵囊的孢子形成情况,计算兔球虫卵囊孢子化率。试验结果表明,2种药物对球虫卵囊孢子化均有抑制效果,但在相同时间、相同浓度时秦皮的抑制作用高于青蒿,且随着药物浓度的增加,对球虫卵囊的孢子化抑制效果也增强。5 d后抑制效果最好的是1.00 g/m L的秦皮,孢子化率为62%;抑制效果最差的是0.25 g/m L的青蒿,孢子化率为85%。In order to study the effects of Artemisia annua and Qinpi Chinese herbal medicines on solutions of rabbit coccidia oocysts in solutions of different mass concentrations,a saturated saline solution floatation method was used to isolate rabbit coccidia oocysts,and the non-sporulated rabbits Coccidia oocysts were placed in Artemisia annua and Qinpi Chinese herbal extracts with different mass concentrations(0.25 g/m L,0.50 g/m L,1.00 g/m L),and cultured continuously at 28°C for 5 days.The spore formation of rabbit coccidia oocysts was observed under a microscope every 24 hours,and the spore formation rate of rabbit coccidia oocysts was counted and calculated.The test results show that both drugs have an inhibitory effect on coccidian oocysts sporulation,but at the same time and at the same concentration,the inhibitory effect of Qinpi is higher than that of Artemisia annua.The effect of sporulation inhibition was also enhanced.After 5 days,the best inhibitory effect was 1.00 g/m L Qinpi,with a sporulation rate of 62%;the worst inhibitory effect was0.25 g/m L Artemisia annua,with a sporulation rate of 85%.

关 键 词：兔球虫卵囊 孢子化率 中草药 

分 类 号：S859.795[农业科学—临床兽医学]