Microbial remediation of a pentachloronitrobenzene-contaminated soil under Panax notoginseng: A field experiment  

作　　者：Ning ZHANG Dong GUO Ye ZHU Xiaomi WANG Lingjia ZHU Fang LIU Ying TENG Peter CHRISTIE Zhengao LI Yongming LUO 

机构地区：[1]College of Resource and Environmental Engineering,Guizhou University,Guiyang 550025(China) [2]Key Laboratory of Soil Environment and Pollution Remediation,Institute of Soil Science,Chinese Academy of Sciences,Nanjing 210008(China) [3]Infinitus(China)Co.,LTD,Jiangmen 529156(China) [4]Institute of Coastal Zone Research,Chinese Academy of Sciences,Yantai 264003(China)

出　　处：《Pedosphere》2020年第4期563-569,共7页土壤圈（英文版）

基　　金：This research was funded by grants from the Major Science and Technique Programs of Yunnan Province,China(No.2016ZF001);the Science and Technique Programs of Yunnan Province,China(No.2017IB038).

摘　　要：Pentachloronitrobenzene(PCNB)is an organochlorine fungicide that is mainly used in the prevention and control of diseases in crop seedlings.Microbial removal is used as a promising method for in-situ removal of many organic pesticides and pesticide residues.A short-term field experiment(1 year)was conducted to explore the potential role of a PCNB-degrading bacterial isolate,Cupriavidus sp.YNS-85,in the remediation of a PCNB-contaminated soil on which Panax notoginseng was grown.The following three treatments were used:i)control soil amended with wheat bran but without YNS-85,ii)soil with 0.15 kg m-2of solid bacterial inoculum(A),and iii)soil with 0.30 kg m-2of solid bacterial inoculum(B).The removal of soil PCNB during the microbial remediation was monitored using gas chromatography.Soil catalase and fluorescein diacetate(FDA)esterase activities were determined using spectrophotometry.In addition,cultivable bacteria,fungi,and actinomycetes were counted by plating serial dilutions,and the microbial biodiversity of the soil was analyzed using BIOLOG.After 1 year of in-situ remediation,the soil PCNB concentrations decreased significantly by 50.3%and 74.2%in treatments A and B,respectively,when compared with the uninoculated control.The soil catalase activity decreased in the presence of the bacterial isolate,the FDA esterase activity decreased in treatment A,but increased in treatment B.No significant changes in plant biomass,diversity of the soil microbial community,or physicochemical properties of the soil were observed between the control and inoculated groups(P<0.05).The results indicate that Cupriavidus sp.YNS-85 is a potential candidate for the remediation of PCNB-contaminated soils under P.notoginseng.

关 键 词：Cupriavidus sp.YNS-85 enzyme activities in-situ remediation PCNB removal SOIL 

分 类 号：X53[环境科学与工程—环境工程]