茶足柄瘤蚜茧蜂滞育和非滞育蛹中与能量代谢相关的差异表达蛋白  

Differentially expressed proteins associated with energy metabolism in diapause and non-diapause pupae of Lysiphlebus testaceipes(Hymenoptera:Braconidae)

作　　者：刘敏 韩海斌[1] 刘爱萍[1] 高书晶[1] 徐林波[1] 岳方正 黄海广 LIU Min;HAN Hai-Bin;LIU Ai-Ping;GAO Shu-Jing;XU Lin-Bo;YUE Fang-Zheng;HUANG Hai-Guang(Grassland Research Institute,Chinese Academy of Agricultural Sciences,Hohhot 010000,China;General Station of Forest and Grassland Diseases and Insect Pests Control,National Forestry and Grassland Bureau,Shenyang 110034,China;Inner Mongolia Academy of Forestry Science,Hohhot 010000,China)

机构地区：[1]中国农业科学院草原研究所,呼和浩特010000 [2]国家林业和草原局森林和草原病虫害防治总站,沈阳110034 [3]内蒙古自治区林业科学研究院,呼和浩特010000

出　　处：《昆虫学报》2020年第6期708-716,共9页Acta Entomologica Sinica

基　　金：国家重点研发项目(2017YFE0104900,2017YFD02010000)。

摘　　要：【目的】本研究旨在从蛋白质组整体层面阐明茶足柄瘤蚜茧蜂Lysiphlebus testaceipes蛹滞育背后的多蛋白调控,重点筛选与能量代谢相关的滞育关联蛋白并分析其功能,有助于更好地理解茶足柄瘤蚜茧蜂蛹滞育的代谢机制。【方法】利用同位素标记相对和绝对定量(isobaric tags for relative and absolute quantification,iTRAQ)技术比较了茶足柄瘤蚜茧蜂滞育蛹与非滞育蛹的蛋白含量;利用GO,KEGG网络数据库等生物信息学方法分析鉴定茶足柄瘤蚜茧蜂滞育蛹与非滞育蛹中差异表达蛋白(differentially expressed proteins,DEPs)。【结果】分析得到茶足柄瘤蚜茧蜂滞蛹与非滞育蛹DEPs有135个,包括滞育蛹中上调表达蛋白有38个,下调表达蛋白有97个。GO和KEGG富集分析表明,与天冬氨酸转运、L-谷氨酸转运、胆碱脱氢酶活性和胆碱生物合成甘氨酸甜菜碱条目以及氧化磷酸化通路相关的蛋白上调表达。【结论】氧化磷酸化通路相关蛋白在茶足柄瘤蚜茧蜂滞育过程中呈显著上调表达,说明能量代谢与该蜂滞育密切相关,并推测氧化磷酸化过程除为滞育蛹提供维持生命基本活动的主要能量外,还提供热能。【Aim】This study aims to elucidate the regulation of multi-proteins behind diapause pupae of Lysiphlebus testaceipes,with focus on the screening of diapause-associated proteins related to energy metabolism and analysis of their functions,and is helpful to better understand the metabolic mechanism of diapause in L.testaceipes.【Methods】The protein contents in diapause and non-diapause pupae of L.testaceipes were compared by isobaric tags for relative and absolute quantification(iTRAQ),and the differentially expressed proteins(DEPs)in diapause and non-diapause pupae of L.testaceipes were analyzed and identified using bioinformatics methods based on GO and KEGG databases.【Results】A total of 135 DEPs in diapause and non-diapause pupae of L.testaceipes,including 38 up-regulated proteins and 97 down-regulated proteins,were found.GO and KEGG enrichment analysis showed that the proteins related to the terms of aspartate transport,L-glutamate transport,choline dehydrogenase activity and glycine betaine biosynthetic process from choline,and the pathway of oxidative phosphorylation were up-regulated.【Conclusion】The proteins associated with oxidative phosphorylation pathway are significantly up-regulated during diapause of L.testaceipes,indicating that energy metabolism is closely related to diapause.It is so speculated that oxidative phosphorylation process provides not only the main energy but also heat energy for the basic activities of diapause pupae.

关 键 词：茶足柄瘤蚜茧蜂 滞育 能量代谢 差异表达蛋白 蛋白含量 iTRAQ 

分 类 号：Q966[生物学—昆虫学]