还少胶囊对奥硝唑诱导的弱精子症模型大鼠生殖功能损伤的保护机制研究  

Mechanisms of Huanshao Capsules protecting the reproductive function in rats with ornidazole-induced asthenozoospermia

作　　者：郭博达 曾银 郭军[4] 韩强[3] 王福[4] 张继伟[4] 余国今[4] 晏斌 刘胜京 高庆和[4] GUO Bo-da;ZENG Yin;GUO Jun;HAN Qiang;WANG Fu;YU Guo-jin;YAN Bin;LIU Sheng-jing;GAO Qing-he(Graduate School,Peking Union Medical College,Beijing 100730,China;Department of Urology,Beijing Hospital/National Center of Gerontology,Beijing 100730,China;Department of Andrology,Beijing Hospital of Traditional Chinese Medicine,Capital Medical University,Beijing 1000103,China;Department of Andrology,Xiyuan Hospital of China Academy of Chinese Medical Sciences,Beijing 100091,China)

机构地区：[1]北京协和医学院研究生院,北京100730 [2]北京医院泌尿外科/国家老年医学中心,北京100730 [3]首都医科大学附属北京中医医院男科,北京100010 [4]中国中医科学院西苑医院男科,北京100091

出　　处：《中华男科学杂志》2020年第5期446-451,共6页National Journal of Andrology

基　　金：国家自然科学基金面上项目(81473527);国家自然科学基金青年项目(81703929)。

摘　　要：目的:探讨还少胶囊对奥硝唑(ORN)诱导的弱精子症模型大鼠生殖功能损伤可能的保护机制。方法:将SD雄性大鼠随机分为4组,每组10只,分别是空白对照组、模型组、还少胶囊组和左卡尼汀组,除空白对照组外,其余3组采用ORN 400 mg/(kg·d)灌胃大鼠28 d,制成弱精子症大鼠模型。并同时连续给药28 d后,处死大鼠,检测大鼠附睾中左卡尼汀的含量,精子浓度、活率,附睾组织中有机阳离子转运子2(OCTN2)mRNA的表达,并观察大鼠睾丸组织病理结构。结果:还少胶囊组、阳性对照药左卡尼汀组与模型组相比,附睾左卡尼汀的含量均可明显提高(6 366.5、6 934.7 mg/L vs 2 880.3 mg/L,P<0.01);改善精子浓度[(46.19±14.23)、(42.25±6.11)×10^6/ml vs(34.58±10.25)×10^6/ml,P<0.01]、活率[(61.34±7.98)%、(61.34±7.98)%vs(42.59±7.54)%,P<0.01];上调附睾OCTN2 mRNA的表达量(27.26、27.15 vs 26.07,P<0.01);同时还少胶囊组能保护ORN造模导致的睾丸生精细胞的病理损伤,使生精细胞在生精小管形态、排列方式、生精细胞的活跃程度上与空白对照组更加接近。结论:还少胶囊对ORN诱导的弱精子症大鼠模型生殖功能损伤具有保护作用,能提高模型大鼠的精子浓度与活率,其机制可能与上调附睾OCTN2 mRNA的表达量、提高附睾左卡尼汀的含量有关。Objective: To explore the possible mechanism of Huanshao Capsules(HSC) protecting the reproductive function in rats with ornidazole-induced asthenozoospermia(AZS). Methods: Forty SD male rats were randomly divided into four groups of equal number, blank control, AZS model control, HSC and L-carnitine(LC) intervention. The AZS model was established in the latter three groups of rats by intragastrical administration of ornidazole at 400 mg/kg/d for 28 days, and meanwhile the animals in the HSC and LC groups were treated by gavage of HSC at 0.31 g/kg/d and LC at 100 mg/kg/d, respectively. Then, all the rats were killed for examination of the LC content, sperm concentration, sperm motility and expression of OCTN2 mRNA in the epididymis and observation of the histopathological changes in the testis tissue. Results: Compared with the AZS model controls, the rats in the HSC and LC groups showed significantly increased LC content(2 880.3 vs 6 366.5 and 6 934.7 mg/L, P < 0.01), sperm concentration([34.58 ± 10.25] vs [46.19 ± 14.23] and [42.25 ± 6.11] ×10^6/ml, P < 0.01), sperm motility([42.59 ± 7.54]% vs [61.34 ± 7.98]% and [61.34 ± 7.98]%, P < 0.01) and expression of OCTN2 mRNA in the epididymis(26.07% vs 27.26% and 27.15%, P < 0.01). The animals of the HSC group exhibited a higher comparability than those of the LC group to the blank controls in the morphology, arrangement and activity of spermatogenic cells. Conclusion: HSC can protect the reproductive function and improve sperm concentration and motility in the model rats with ornidazole-induced AZS, which may be associated with its abilities of up-regulating the expression of OCTN2 mRNA and increasing the LC content in the epididymis.

关 键 词：还少胶囊 弱精子症 奥硝唑 左卡尼汀 有机阳离子转运子2 大鼠 

分 类 号：R698.2[医药卫生—泌尿科学]