全基因组测序在结核分枝杆菌研究中的应用  

Application of whole genome sequencing in research of Mycobacterium tuberculosis

作　　者：张洁[1,2] 任怡宣[1,2] 潘丽萍 张宗德[1] ZHANG Jie;REN Yi-xuan;PAN Li-ping;ZHANG Zong-de(Beijing Key Laboratory of Drug-resistant Tuberculosis Research,Institute of Tuberculosis and Thoracic Tumor,Beijing Chest Hospital,Capital Medical University,Beijing 101149,China;Central Laboratory of Beijing Research Institute for Tuberculosis Control,Beijing 100035,China;不详)

机构地区：[1]首都医科大学附属北京胸科医院,北京市结核病胸部肿瘤研究所,耐药结核病研究北京市重点实验室,101149 [2]北京结核病控制研究所中心实验室

出　　处：《中国防痨杂志》2020年第7期737-740,共4页Chinese Journal of Antituberculosis

基　　金：“十三五”国家科技重大专项(2017ZX10201301004);北京市医院管理局“登峰”人才培养计划(DFL20181601)。

摘　　要：全基因组测序技术已经广泛应用于结核分枝杆菌的研究中,包括结核分枝杆菌谱系或亚种鉴定、区分患者的发病模式,以及识别传染源、超级传播者和确定传播方向,以及用于结核分枝杆菌的耐药性预测和微进化研究等,在临床和公共卫生领域发挥了很重要的作用。即便如此,目前还存在很多问题需要解决。作者对全基因组测序技术在结核分枝杆菌研究中的相关内容、应用成果及进展进行综述。Whole genome sequencing has been widely used in the research of Mycobacterium tuberculosis(M.tuberculosis),including classification of M.tuberculosis lineages or subspecies,differentiation of disease patterns,identification of putative source cases and super-spreaders,determination of the transmission chains and their directions,as well as prediction of drug resistance of M.tuberculosis and detection of microevolution of M.tuberculosis.Although the whole genome sequencing has played an important role in clinical and public health applications,many problems are still existed and should be resolved in future.Herein,the current research directions and achievements of whole genome sequencing are reviewed,and the future applications are prospected.

关 键 词：分枝杆菌 结核 全基因组关联研究 高通量核苷酸测序 分子诊断技术 综述 

分 类 号：R378.911[医药卫生—病原生物学]