猫杯状病毒GX2019的分离鉴定及全基因克隆遗传进化分析  

Isolation and identification of feline calicivirus GX2019 and genetic evolution analysis of whole gene cloning

作　　者：王浩[1,2] 秦树英 刘金凤 白安斌 覃绍敏 吴健敏 WANG Hao;QIN Shu-ying;LIU Jin-feng;BAI An-bin;QIN Shao-min;WU Jian-min(College of Animal Science and Technology,Guangxi University,Nanning 530004,China;Guangxi Veterinary Research Institute of Veterinary Biotechnology,Nanning 530001,China)

机构地区：[1]广西大学动物科学技术学院,广西南宁530004 [2]广西壮族自治区兽医研究所广西兽医生物技术重点实验室,广西南宁530001

出　　处：《中国兽医科学》2020年第7期880-885,共6页Chinese Veterinary Science

基　　金：国家重点研发计划项目(2016YFD0501000);广西基本科研业务费专项(桂科专项17-4;桂科专项18-4)。

摘　　要：从南宁某宠物医院疑似感染猫杯状病毒(Feline calicivirus,FCV)猫鼻咽拭子中,利用F81细胞分离1株FCV后,RT-PCR和电镜鉴定,测定TCID50和理化性质。同时利用RT-PCR分段扩增病毒全基因序列,进行同源性及遗传进化分析。结果显示,FCV GX2019分离株的TCID50为1×10-8.67/0.1 mL,基因组全长为7687 bp,与FCV CH-JL4(长春株)毒株核苷酸序列相似性最高,为85.3%,属于同一分支。氨基酸分析表明,与国内FCV疫苗株255、国外FCV疫苗株F9、FCV跛行综合征株2280及FCV GX01分离株氨基酸变异率分别为46.7%、26.7%、53.3%、40.0%,其中B细胞抗原识别表位中氨基酸位点发生了改变。F81 cells were used to isolate a FCV strain,from a nasopharyngeal swab of a cat suspected of having feline calicivirus(FCV)in a pet hospital in Nanning,and the FCV was identified by RT-PCR and electron microscopy,and then the TCID50 and physicochemical properties of the were determined.The complete sequence of the strain was amplified in RT-PCR,and similarity and genetic evolution were analyzed.The results showed that the FCV’s TCID50 was 1×10-8.67/0.1 mL and the FCV’s genome was 7687 bp in size.The FCV had the highest similarity with China’s CH-JL4 strain(Changchun isolates),which was 85.3%.The analysis of the amino acid sequences showed that the mutation rates of the FCV amino acid with domestic FCV vaccine 255 strains,foreign FCV vaccine F9 strains,FCV claudication syndrome 2280 strains,and FCV GX01 strains were 46.7%,26.7%,53.3%and 40.0%,respectively.The important amino acid positions in the B cell antigen recognition epitopes were changed.

关 键 词：猫杯状病毒 分离鉴定 全基因克隆 遗传进化分析 

分 类 号：S852.659.6[农业科学—基础兽医学]