Genetic Diversity, Antimicrobial Resistance, and Virulence Genes of Aeromonas Isolates from Clinical Patients, Tap Water Systems, and Food  

作　　者：MENG Shuang WANG Yong Lu LIU Chen Geng YANG Jing YUAN Min BAI Xiang Ning JIN Dong LIANG Jun Rong CUI Zhi Gang LI Juan 

机构地区：[1]State Key Laboratory of Infectious Disease Prevention and Control,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 10026,China [2]Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases,Zhejiang 310058,Hangzhou,China [3]Ma'anshan Center for Disease Control and Prevention,Ma'anshan 243031,Anhui,China [4]Clinical Laboratoty of Xuanwu Hospital,Captital Medcial University',Beijing 100053,China

出　　处：《Biomedical and Environmental Sciences》2020年第6期385-395,共11页生物医学与环境科学（英文版）

基　　金：supported by the National Natural Science Foundation of China grant numbers NSFC 81861138053 and NSFC 31761133004。

摘　　要：Objective This study aimed to evaluate the genetic diversity,virulence,and antimicrobial resistance of Aeromonas isolates from clinical patients,tap water systems,and food.Methods Ninety Aeromonas isolates were obtained from Ma’anshan,Anhui province,China,and subjected to multi-locus sequence typing(MLST)with six housekeeping genes.Their taxonomy was investigated using concatenated gyr B-cpn60 sequences,while their resistance to 12 antibiotics was evaluated.Ten putative virulence factors and several resistance genes were identified by PCR and sequencing.Results The 90 Aeromonas isolates were divided into 84 sequence types,80 of which were novel,indicating high genetic diversity.The Aeromonas isolates were classified into eight different species.PCR assays identified virulence genes in the isolates,with the enterotoxin and hemolysin genes act,aer A,alt,and ast found in 47(52.2%),13(14.4%),22(24.4%),and 12(13.3%)of the isolates,respectively.The majority of the isolates(≥90%)were susceptible to aztreonam,imipenem,cefepime,chloramphenicol,gentamicin,tetracycline,and ciprofloxacin.However,several resistance genes were detected in the isolates,as well as a new mcr-3 variant.Conclusions Sequence type,virulence properties,and antibiotic resistance vary in Aeromonas isolates from clinical patients,tap water systems,and food.

关 键 词：AEROMONAS Multi-locus sequence typing Multidrug resistance Virulence gene Antimicrobial resistance gene 

分 类 号：R446.5[医药卫生—诊断学]