生物大分子相对分子质量估算法  

Discussion on the Relative Molecular Weight Estimation for Biomacromolecules

作　　者：王志鹏 蒋振雄[4,5] 车子良 马新雨 汪璐 姜汉杰 王鹏 郑少庸[7] WANG Zhipeng A;JIANG Zhen-Xiong;CHE Zi-Liang;MA Xin-Yu;WANG Lu;JIANG Han-Jie;WANG Peng;ZHENG Shao-Yong(Division of Genetics,Department of Medicine,Brigham and Women's Hospital,Department of Biological Chemistry and Molecular Pharmacology,Harvard Medical School,Boston,MA 02115,USA;Department of Chemistry,Texas A&M University,College Station,TX 77840,USA;Department of Chemistry,Tsinghua University,Beijing 100084,China;Department of Plant and Pathology and Microbiology,Texas A&M University,College Station,TX 77840,USA;Department of Biology,Texas A&M University,College Station,TX 77840,USA;Burn Surgery,The First Affiliated Hostital,Sun Yat-sen University,Guangzhou 510080,China;Department of Food Science,Jinshan College of Fujian Agriculture and Forestry University,Fujian 350000,China)

机构地区：[1]Division of Genetics,Department of Medicine,Brigham and Women's Hospital,Department of Biological Chemistry and Molecular Pharmacology,Harvard Medical School,Boston,MA 02115,USA [2]Department of Chemistry,Texas A&M University,College Station,TX 77840,USA [3]清华大学化学系,北京100084 [4]Department of Plant and Pathology and Microbiology,Texas A&M University,College Station,TX 77840,USA [5]Department of Biology,Texas A&M University,College Station,TX 77840,USA [6]中山大学附属第一医院烧伤外科,广东广州510080 [7]福建农林大学金山学院食品科学系,福建福州350000

出　　处：《化学教育（中英文）》2020年第12期91-98,共8页Chinese Journal of Chemical Education

摘　　要：生物大分子相对分子质量的计算在科学定量分析中非常重要,但并非所有情况都可进行精确的计算,而需要基于聚合物大小进行估算。通过回归生物大分子之高分子本质,系统性地分析以单体平均相对分子质量出发进行估算的方法。以蛋白质和单双链脱氧核糖核酸(DNA)及核糖核酸(RNA)为例进行分析,旨在探讨和比较基于单体的相对分子质量估计法对不同的生物大分子的可能最大误差。同时指出一个关于双链DNA常被忽略的知识。Relative molecular weight calculation plays a nonnegligible role in the scientific quantitative analysis. However, not all calculations can be made with high accuracy: under certain circumstances the length-based estimation is the only way for relative molecular weight estimation. This paper systemically introduces a feasible method based on the averaged relative molecular weight of monomers. Single-stranded and double-stranded deoxyribonucleic acid, ribonucleic acid, and proteins are selected here for analysis in order to elucidate and compare the maximal biases based on this methodology. Meanwhile, one rarely explored tip about double stranded DNA is mentioned.

关 键 词：生物大分子 相对分子质量 碱基 脱氧核糖核酸 

分 类 号：Q51[生物学—生物化学] Q52