荧光定量PCR对两种不同动物皮膜血吸虫尾蚴检测结果的比较  

Comparison of test results of Schistosomiasis cercariae from two different animal membranes by quantitative fluorescence PCR

作　　者：彭国华 石少东 邓凤林 钱科 胡主花 余章科 冯小武 李石柱[4] 胡绍良 余晴[4] PENG Guohua;SHI Shaodong;DENG Fenglin;QIAN Ke;HU Zhuhua;YU Zhangke;FENG Xiaowu;LI Shizhu;HU Shaoliang;YU Qing(The Collaboration Unit for Field Epidemiology of State Key Laboratory for Infectious Disease Prevention and Control,Jiangxi Provincal Key Laboratory of Animal-origin and Vector-borne Diseases,Nanchang Center for Disease Control and Prevention.Nanchang,Jiangxi 330038,China;Shenzhen Combined Biotech Co.,Ltd.,Shenzhen,Guangdong 518057,China;Nanchang Xinjian District Schistosomiasis Control Station,Nanchang,Jiangxi 330100,China;National Institute of Parasitic Diseases,China CDC,WHO Collaborating Centre for Tropical Diseases,National Center for International Research on Tropical Diseases Key Laboratory of Parasite and Vector Biology,Ministry of Health,Shanghai 200025,China)

机构地区：[1]传染病预防控制国家重点实验室研究基地,江西省动物源与媒介生物性传染病重点实验室,南昌市疾病预防控制中心,江西南昌330038 [2]深圳市康百得生物科技有限公司,广东深圳518057 [3]南昌市新建区血吸虫病防治站,江西南昌330100 [4]中国疾病预防控制中心寄生虫病预防控制所,世界卫生组织热带病合作中心,国家热带病研究中心,卫生部寄生虫病原与媒介生物学重点实验室,上海200025

出　　处：《中国热带医学》2020年第7期617-620,636,共5页China Tropical Medicine

摘　　要：目的比较两种不同动物皮膜获取血吸虫尾蚴效果,为动物皮膜优选提供参考。方法分别制作添加苯甲酸钠(A膜)与未添加苯甲酸钠(B膜)两种动物皮膜,将尾蚴设置成1~10条、11~99条、≥100条3个浓度组,采用荧光定量PCR法,对两种动物皮膜获取尾蚴效果进行综合比较。结果实验室测试结果显示,尾数1~10条组,A膜和B膜的血吸虫尾蚴检出率均为77.78%,其余两个组检出率均为100%,总检出率均为90.48%。A膜和B膜的血吸虫尾蚴检出率比较差异无统计学意义(P>0.05)。对模拟现场的A膜和B膜的血吸虫尾蚴的检出率均为5.00%,两者比较差异无统计学意义(P>0.05)。结论添加与未添加苯甲酸钠动物皮膜获取尾蚴效果一致,但因苯甲酸钠具有防腐剂作用,可用于动物皮膜的长时间保存,因此推荐含有苯甲酸钠的仿生膜用于现场连续使用。ObjectiveTo compare influence of two kinds of animal membrane on obtaining Schistosoma cercariae,aiming at providing reference for optimal selection of animal membranes.MethodsTwo kinds of animal membrane, namely membrane with sodium benzoate and without sodium benzoate(hereinafter referred to as membrane A and membrane B) were prepared, while 3 groups were set according to the number of cercariae(1-10, 11-99, ≥100 cercariae). Quantitative fluorescent PCR(QF-PCR) was used for comprehensive comparison of influence on cercariae acquisition between two kinds of membrane.ResultsLaboratory test results showed that detection rates of Schistosoma cercariae from membrane A and membrane B were both 77.78% in the group of 1-10 cercariae and both 100% in the other two groups. The total detection rates were both 90.48%.There was no statistical significance observed in the comparison of detection rate of Schistosoma cercariae from membrane A and membrane B(P>0.05). Besides, detection rates of Schistosoma cercariae from simulated on-the-spot membrane A and B were both 5.00%, showing no statistical significance either(P>0.05).ConclusionThere is no difference shown in influence on cercariae acquisition between membrane with and without sodium benzoate. As sodium benzoate can also play the role of preservative, it can be used in long-term preservation of animal membranes. Thus, bionic membranes containing sodium benzoate are suggested for continuous use on site.

关 键 词：日本血吸虫 尾蚴 仿生动物皮膜 

分 类 号：R383.2+4[医药卫生—医学寄生虫学]