考马斯亮蓝R250共振光散射光谱法测定壳聚糖含量  

Determination of Chitosan Content by Coomassie Brilliant Blue R250 Resonance Light Scattering Spectroscopy

作　　者：许钟元 袁莉 李银华 王振 余梦婷 马卫兴 陈丽 XU Zhong-yuan;YUAN Li;LI Yin-hua;WANG Zhen;YU Meng-ting;MA Wei-xing;CHEN Li(School of Marine Life and Fisheries,Jiangsu Ocean University,Lianyungang 222005,China;School of Pharmacey,Jiangsu Ocean University,Lianyungang 222005,China;School of Chemical Engineering,Jiangsu Ocean University,Lianyungang 222005,China;Jiangsu Key Laboratory of Marine Pharmaceutical School Screening,Jiangsu Ocean University,Lianyungang 222005,China;Jiangsu Academy of Marine Resources Development,Jiangsu Ocean University,Lianyungang 222005,China)

机构地区：[1]江苏海洋大学海洋生命与水产学院,江苏连云港222005 [2]江苏海洋大学药学院,江苏连云港222005 [3]江苏海洋大学江苏海洋大学化学工程学院,江苏连云港222005 [4]江苏海洋大学江苏省海洋药物筛选重点实验室,江苏连云港222005 [5]江苏海洋大学江苏省海洋资源开发研究院,江苏连云港222005

出　　处：《化学试剂》2020年第7期832-835,共4页Chemical Reagents

基　　金：江苏海洋大学一流专业(2019)建设项目(序号12);淮海工学院研究生创新计划项目(KYCX19-2299)。

摘　　要：壳聚糖为碱性高分子多糖,可广泛应用于各个领域,因此壳聚糖的含量测定至关重要。研究表明,壳聚糖在pH 2.4的Clark-Lubs缓冲溶液中形成质子化的壳聚糖阳离子,再与阴离子染料考马斯亮蓝R250形成离子缔合物,使体系的共振光散射强度显著增强。在325 nm处共振光强度增强值ΔI与壳聚糖浓度在0.1~0.8 mg/L内呈线性关系,检出限(3s/k)为0.012 mg/L。方法用于壳聚糖胶囊的分析,测定值与变色酸2B褪色分光光度法结果一致。Chitosan is a basic polymer polysaccharide,which can be widely used in various fields,so the determination of chitosan content is very important.The work shows that chitosan forms protonated chitosan cations in Clark-Lubs buffer solution at pH 2.4,which bonded with coomassie brilliant blue R250(anionic dye)could significantly enhance the intensity of resonance light.The resonance light intensity enhancement valueΔI has a linear relationship with the concentration of chitosan within 0.1~0.8 mg/L,and the detection limit(3s/k)was 0.012 mg/L.The method was used for the analysis of chitosan capsules,and the measured values were consistent with the results of discoloring spectrophotometry of chromotrope 2 B.

关 键 词：壳聚糖 考马斯亮蓝R250 共振光散射(RLS) 离子缔合反应 含量测定 

分 类 号：O-31[理学]