风信子不同花色品种花青素苷含量及相关基因表达分析  

Analysis of Anthocyanin Content and Related Gene Expression in Different Varieties of Hyacinthus orientalis

作　　者：李霞[1] 吴钰滢 封晔 Li Xia;Wu Yuying;Feng Ye(Urban Construction School,Beijing City University,Beijing,100083;School of Landscape Architecture,Beijing Forestry University,Beijing,100083)

机构地区：[1]北京城市学院城市建设学部,北京100083 [2]北京林业大学园林学院,北京100083

出　　处：《分子植物育种》2020年第14期4562-4571,共10页Molecular Plant Breeding

基　　金：大北农青年教师科研基金(15ZK008)资助。

摘　　要：风信子为百合科风信子属多年生球根花卉,其花色变异非常丰富。本研究以8个不同花色的风信子品种为研究材料,从生理和分子角度分析了风信子花色差异的机制,结果显示所测风信子品种花瓣中均不含类胡萝卜素,粉、紫、红、蓝和黑色品种中含有花青素。其中黑色品种‘黑巴克’花瓣中的花青素含量最高。粉、紫和蓝色品种中均只含有天竺葵素衍生物(Pg),‘黑巴克’中含有飞燕草素衍生物(Dp)和芍药素衍生物(Pn)。通过转录组测序得到了‘黑巴克’中花青素代谢结构基因和主要调节基因的序列,采用qRT-PCR分析其在白、红、蓝和黑色四个色系品种中的表达模式。研究结果表明,HoF3H、HoMYB4、HoMYB6和HoMYB7为影响风信子花青素积累的关键基因,HoDFR2是红、蓝色花中特异表达的基因,有可能和Pg合成相关,而HoF3’5’H1、HoFOMT2是影响黑色花中芍药色素合成的关键基因。本研究结果对于深入理解观赏植物花色形成和分支机制具有一定的理论意义。Hyacinthus orientalis is a perennial herbaceous bulb flower of the lily family hyacinth with rich flower color variation. In this study, eight different color hyacinth varieties were used as research materials. The mechanism of hyacinth flower color differences from the physiological and molecular perspective was analyzed. The results showed that none of the measured hyacinth petals contained carotenoids, anthocyanins were found in pink, purple,red, blue and black varieties. Among which the black variety ’Black Baccara’ had the highest anthocyanin content in the petals. Pink, purple and blue varieties contained Pg. ’Black Baccara’contained Dp and Pn derivatives(Pn).Sequences of anthocyanin metabolic structural genes and major regulatory genes in ’Black Baccara’ were obtained by transcriptome sequencing. Analysis of expression patterns in four color systems of white, red, blue and black were analyzed by qRT-PCR. The results showed that HoF3 H, HoMYB4, HoMYB6 and HoMYB7 were the key genes affecting the accumulation of hyacinth anthocyanins. HoDFR2 specifically expressed in red and blue flowers, which may be related to Pg synthesis. HoF3’5’H1 and HoFOMT2 were the key genes that affected the synthesis of peony pigment in black flowers. This study had certain theoretical significance for the in-depth understanding of the flower color formation and branching mechanism of ornamental plants.

关 键 词：风信子(Hyacinthus orientalis L.) 花青素苷 基因表达 

分 类 号：S682.29[农业科学—观赏园艺]