不同外源物质对镉砷复合污染胁迫下油菜生理指标和镉砷积累的影响  

Effects of spraying different exogenous substances on physiological indices and cadmium and arsenic accumulation in rape under combined pollution of cadmium and arsenic

作　　者：张盛楠 黄亦玟 陈世宝[3] 王萌[3] 黄益宗 韩廿 ZHANG Sheng-nan;HUANG Yi-wen;CHEN Shi-bao;WANG Meng;HUANG Yi-zong;HAN Nian(Agro-Environmental Protection Institute,Ministry of Agriculture and Rural Affairs,Tianjin 300191,China;Beijing 101 Middle School,Beijing 100091,China;Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]农业农村部环境保护科研监测所,天津300191 [2]北京一零一中学,北京100091 [3]中国农业科学院农业资源与农业区划研究所,北京100081

出　　处：《生态学杂志》2020年第7期2214-2222,共9页Chinese Journal of Ecology

基　　金：国家重点研发计划项目(2017YFD0801500);国家科技支撑计划项目(2015BAD05B02)资助。

摘　　要：为缓解重金属对作物的胁迫,降低重金属对作物的毒害作用,通过叶面喷施比较不同外源物质茉莉酸(JA)、褪黑素(MT)、亚精胺(SPD)和2,4-表油菜素内酯(EBL)对Cd、As胁迫下油菜生理指标及吸收积累Cd、As的影响。结果表明:喷施200μmol·L-1的JA和MT均显著提高油菜叶片的叶绿素SPAD值,而喷施20μmol·L-1的EBL显著降低油菜叶片的叶绿素SPAD值;与CK处理相比,喷施500μmol·L-1SPD和20μmol·L-1EBL分别降低油菜叶片中MDA含量41.0%和52.3%,同时EBL处理提高油菜叶片SOD活性88.2%;喷施200μmol·L-1MT导致油菜地上部Cd含量比CK处理降低27.8%,而喷施200μmol·L-1的JA和20μmol·L-1的EBL却分别提高油菜地上部As含量159.8%和136.8%;JA、MT、SPD和EBL在某种程度上均缓解了Cd、As复合污染对油菜的胁迫,为重金属污染农田防治提供修复材料。To alleviate the stress from heavy metals and reduce their toxic effects on crops,the effects of jasmonic acid(JA),melatonin(MT),spermidine(SPD),and 2,4-epibrassinolide(EBL)on physiological indices,uptake and accumulation of Cd and As in rape under combined Cd and As stress were examined.The results showed that spraying JA and MT at a concentration of 200μmol·L-1 significantly increased and spraying 20μmol·L-1 EBL significantly reduced the chlorophyll SPAD value of rape leaves.Spraying 500μmol·L-1 SPD and 20μmol·L-1 EBL significantly reduced the MDA content in rape leaves by 41.0%and 52.3%,respectively,whereas 20μmol·L-1 EBL treatment significantly increased the SOD activity of rape leaves by 88.2%.Spraying 200μmol·L-1 MT significantly decreased Cd concentration in the shoots of rape by27.8%compared with CK treatment,while spraying 200μmol·L-1 JA and 20μmol·L-1 EBL significantly increased As concentration in the shoots of rape by 159.8%and 136.8%,respectively.Our results suggested that JA,MT,SPD and EBL can alleviate the stress of combined Cd and As pollution on rape,providing remediation materials for controlling heavy metal pollution in croplands.

关 键 词：Cd As 土壤 茉莉酸 褪黑素 亚精胺 2 4-表油菜素内酯 

分 类 号：S565.4[农业科学—作物学] X53[环境科学与工程—环境工程]