赤潮藻米氏凯伦藻(Karenia mikimotoi)藻际细菌群落及其原位抑藻效应  

Microbial community in phycosphere of red-tide alga Karenia mikimotoi and in situ algicidal effect

作　　者：丁宁[1] 郑宁宁 王仁君[1] 孙丽 李晨 高配科[1] DING Ning;ZHENG Ning-ning;WANG Ren-jun;SUN Li;LI Chen;GAO Pei-ke(Cllege of Life Science,Qufiu Normal University,Qufu 273165,Shandong,China)

机构地区：[1]曲阜师范大学生命科学学院,山东曲阜273165

出　　处：《生态学杂志》2020年第7期2356-2363,共8页Chinese Journal of Ecology

基　　金：国家自然科学基金项目(31200400和31500414)资助。

摘　　要：采用16S rRNA高通量测序技术分析了赤潮藻米氏凯伦藻藻际细菌群落组成,探究了原位营养刺激下藻际细菌对米氏凯伦藻生长的抑制作用。结果表明:米氏凯伦藻藻际环境以Proteobacteria为主(α-Proteobacteria 48.1%,γ-Proteobacteria 8.6%),其次Sphingobacteria 22.4%,Cyanobacteria 10.8%。通过加混合抗生素获得了米氏凯伦藻无菌培养体系,验证了所加营养液对藻的生长无明显影响。向米氏凯伦藻培养体系中添加体积比5%的2216E培养基、甘油和柠檬酸钠时,米氏凯伦藻的生长受到显著抑制,抑制率分别为99.6%、96.0%和91.5%。对2216E刺激下藻际细菌群落变化进行分析表明,α-Proteobacteria丰度增至58.7%,其中Rhodobacteraceae由8.7%增至22.3%,Thalassobaculum由2.7%增至13.3%,γ-Proteobacteria增至13.3%,其中Pseudomonas由2.4%增至4.9%。米氏凯伦藻藻际环境存在着丰富的微生物类群,适量外部营养刺激可以实现藻际细菌原位抑藻效应。We analyzed microbial community composition in phycosphere of red-tide alga Karenia mikimotoi by 16 S rRNA high-throughput sequencing,and investigated the inhibitory effect of the phycosphere microorganisms on the growth of K.mikimotoi with in situ nutritive stimulation.The results showed that Proteobacteria was the dominant bacterial population in phycosphere of K.mikimotoi,with an abundance of 48.1%forα-Proteobacteria and 8.6%forγ-Proteobacteria.Sphingobacteria and Cyanobacteria accounted for 22.4%and 10.8%,respectively.Axenic culture of K.mikimotoi was obtained via treatment of the combined antibiotics.Nutrient addition did not affect the growth of K.mikimotoi.When 2216 E culture medium,glycerol and sodium citrate in5%volume ratio were added to cultivation system of K.mikimotoi,their growth was inhibited,with an inhibition rate of 99.6%,96.0%and 91.5%,respectively.The response of phycosphere microbial community to 2216 E stimulation showed that the abundance ofα-Proteobacteria increased to 58.7%(within which Rhodobacteraceae increased from 8.7%to 22.3%and Thalassobaculum increased from 2.7%to 13.3%),and that ofγ-Proteobacteria increased to 13.3%(within which Pseudomonas increased from 2.4%to 4.9%).There were diverse microbial populations in phycosphere of K.mikimotoi,whereas appropriate amount of nutrient addition would provide the in situ alga-inhibiting effect of phycosphere bacteria.

关 键 词：米氏凯伦藻 藻际环境 抑藻细菌 16S rRNA 原位抑藻 

分 类 号：X55[环境科学与工程—环境工程]