HPLC-PDA法同时测定降脂通便胶囊中6种成分的含量  

Simultaneous determination of 6 constituents in Jiangzhi Tongbian capsules by HPLC-PDA

作　　者：田雁钰 张桂平 TIAN Yan-yu;ZHANG Gui-ping(Nanyang Institute for Food and Drug Control,Nanyang Henan 473061)

机构地区：[1]南阳市食品药品检验所,河南南阳473061

出　　处：《中南药学》2020年第7期1164-1167,共4页Central South Pharmacy

摘　　要：目的建立HPLC-PDA法同时测定降脂通便胶囊中大黄素、大黄酚、人参皂苷Rg_1、人参皂苷Re、人参皂苷Rb_1、桂皮醛的含量。方法色谱柱为Welch Materials Eclipse XB-C_(18)(250 mm×4.6 mm,5 μm),流动相为乙腈(A)-0.1%磷酸水溶液(B)(梯度洗脱),检测波长为254 nm(大黄素、大黄酚)、203 nm(人参皂苷Rg_1、人参皂苷Re、人参皂苷Rb_1)、290 nm(桂皮醛),流速为1.0 mL·min^(-1),柱温为30℃,进样量为10 μL。结果 6种成分在各自对应的线性浓度范围内与峰面积线性关系良好(r均≥0.9991);平均回收率在97.8%~99.0%,RSD均在0.72%~1.2%(n=6)。结论该方法简便准确,结果重现性好,可用于降脂通便胶囊的质量控制。Objective To determine the content of emodin,chrysophanol,ginsenosidein Rg1,ginsenosidein Re,ginsenosidein Rb1,and cinnamaldehyde in Jiangzhi Tongbian capsules by HPLC-PDA.Methods Welch Materials Eclipse XB-C18 column(250 mm×4.6 mm,5μm)was used.The mobile phase was acetonitrile(A)-0.1%phosphoric(B)with gradient elution.The detection wavelength was 254 nm for emodin and chrysophanol,203 nm for ginsenosidein Rg1,ginsenosidein Re,and ginsenosidein Rb1,290 nm for cinnamaldehyde.The flow rate was 1.0 mL·min-1.The column temperature was 30℃.The sample size was 10μL.Results All the 6 components showed good linearity.The average recovery was between 97.8%-99.0%,RSD was between 0.72%-1.2%(n=6).Conclusion The method is simple,accurate,and reproducible,and can be used for the quality control of Jiangzhi Tongbian capsules.

关 键 词：HPLC-PDA法 降脂通便胶囊 大黄素 大黄酚 人参皂苷Rg_1 人参皂苷RE 人参皂苷Rb_1 桂皮醛 

分 类 号：R917[医药卫生—药物分析学] R285[医药卫生—药学]