HPLC法测定儿感退热宁口服液中连翘酯苷B、连翘酯苷A、连翘苷、去芹糖桔梗皂苷E、桔梗皂苷E、桔梗皂苷D3、青蒿乙素和青蒿酸  

Determination of sythoside B, forsythoside A, forsythin, deapi-platycoside E, platycoside E, platycodin D, arteannuin B, and artemisinic acid in Ergan Tuirening Oral Liquid by HPLC

作　　者：李季梅 唐铖 高森[3] LI Ji-mei;TANG Cheng;GAO Sen(Department of Pharmacy,TianJin Ninghe Hospital,Tianjin 301500,China;Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics(Theranostics),College of Pharmacy,Tianjin Medical University,Tianjin 300070,China;Department of Pharmacy,General Hospital,Tianjin Medical University,Tianjin 300052,China)

机构地区：[1]天津市宁河区医院药剂科,天津301500 [2]天津医科大学药学院天津市临床药物关键技术重点实验室,天津300070 [3]天津医科大学总医院药剂科,天津300052

出　　处：《现代药物与临床》2020年第6期1080-1084,共5页Drugs & Clinic

摘　　要：目的建立HPLC法同时测定儿感退热宁口服液中连翘酯苷B、连翘酯苷A、连翘苷、去芹糖桔梗皂苷E、桔梗皂苷E、桔梗皂苷D3、青蒿乙素和青蒿酸。方法 Agilent TC-C18色谱柱(250 mm×4.6 mm,5μm);流动相为乙腈–0.2%磷酸水溶液,梯度洗脱;检测波长:275 nm(0~21.0 min检测连翘酯苷B、连翘酯苷A、连翘苷)和210 nm(21.0~55.0 min检测去芹糖桔梗皂苷E、桔梗皂苷E、桔梗皂苷D3、青蒿乙素、青蒿酸);柱温25℃;体积流量0.9 mL/min;进样量:10μL。结果连翘酯苷B、连翘酯苷A、连翘苷、去芹糖桔梗皂苷E、桔梗皂苷E、桔梗皂苷D3、青蒿乙素和青蒿酸分别在3.47~69.40μg/mL(r=0.999 5)、18.78~375.60μg/mL(r=0.999 2)、6.64~132.80μg/mL (r=0.999 7)、2.57~51.40μg/mL (r=0.999 5)、4.86~97.20μg/mL(r=0.999 3)、4.23~84.60μg/mL(r=0.999 8)、0.79~15.80μg/mL(r=0.999 1)、2.16~43.20μg/mL(r=0.999 9)线性关系良好,平均加样回收率分别为98.76%、100.12%、99.34%、97.57%、98.29%、99.25%、96.87%、98.02%,RSD值分别为1.53%、0.89%、0.91%、1.38%、1.02%、0.83%、1.26%、1.15%。结论所建立的方法结果准确,可用于儿感退热宁口服液中多成分的质量控制研究。Objective To develop an HPLC method for determination of sythoside B, forsythoside A, forsythin, deapi-platycoside E, platycoside E, platycodin D3, arteannuin B, and artemisinic acid in Ergan Tuirining Oral Liquid. Methods The separation was performed on an Agilent TC-C18 column(250 mm × 4.6 mm, 5 μm) with the mobile phase consisted of acetonitrile and 0.2% phosphoric acid solution for gradient elution. The detection wavelength were set at 275 nm for forsythoside B, forsythoside A, and forsythin, and 210 nm for deapi-platycoside E, platycoside E, platycodin D3, arteannuin B, and artemisinic acid. The column temperature was set at 25 ℃, and the flow rate was 0.9 mL/min with injection of 10 μL. Results Forsythoside B, forsythoside A, forsythin, deapi-platycoside E, platycoside E, platycodin D3, arteannuin B, and artemisinic acid showed good linear relationships in the ranges of 3.47 — 69.40 μg/mL(r =0.999 5), 18.78 — 375.60 μg/mL(r =0.999 2), 6.64 — 132.80 μg/mL(r =0.999 7), 2.57 — 51.40 μg/mL(r =0.999 5), 4.86 — 97.20 μg/mL(r =0.999 3),4.23 — 84.60 μg/mL(r =0.999 8), 0.79 — 15.80 μg/mL(r =0.999 1),2.16 — 43.20 μg/mL(r ==0.999 9). Their average recoveries(RSDs) were 98.76%(1.53%), 100.12%(0.89%), 99.34%(0.91%), 97.57%(1.38%), 98.29%(1.02%), 99.25%(0.83%), 96.87%(1.26%), and 98.02%(1.15%), respectively. Conclusion The HPLC method is accurate which can be used for the quality control of Ergan Tuirining Oral Liquid.

关 键 词：儿感退热宁口服液 连翘酯苷B 连翘酯苷A 连翘苷 去芹糖桔梗皂苷E 桔梗皂苷E 桔梗皂苷D3 青蒿乙素 青蒿酸 高效液相色谱 

分 类 号：R286.02[医药卫生—中药学]