前列腺炎合并不育症患者弹性蛋白酶、miR-551b水平及诊断疾病价值  

The expression levels of elastase and miR-551b of infertility men with prostatitis and their diagnostic value

作　　者：熊标[1] 朱里 卢童[1] 周治军[1] XIONG Biao;ZHU Li;LU Tong;ZHOU Zhijun(The First People’s Hospital of Tianmen,Hubei Province,431700)

机构地区：[1]湖北省天门市第一人民医院,431700

出　　处：《中国计划生育学杂志》2020年第7期1095-1097,共3页Chinese Journal of Family Planning

摘　　要：目的:分析前列腺炎合并不育症患者前列腺按摩液(EPS)中弹性蛋白酶(NE)、miR-551b表达水平及其对疾病的诊断价值。方法:2017年7月—2019年5月本院收治的前列腺炎合并不育症患者60例作为观察组,同期选取60例健康志愿者为对照组。比较两组男性EPS中NE和miR-551b表达水平及其相关性,以及水平对前列腺炎合并不育症诊断效能。结果:NE水平[1472.25(812.23,1735.65)]高于对照组[156.38(76.35,226.52)],miR-551b[5.13(2.34,13.47)]低于对照组[22.31(8.64,42.23)](P<0.05)。Spearman秩相关分析显示,观察组NE与miR-551b表达呈显著负相关(r=-0.874,P<0.05)。NE和miR-551b联合诊断前列腺炎合并不育症的敏感度(79.2%)、特异度(81.5%)和准确性(84.3%)均高于单一检测,AUC(0.786)高于单独检测(P<0.05)。结论:NE和miR-551b联合检测EPS具有较好的诊断价值,有望成为诊断前列腺炎患者不育症的分子标志物。Objective:To analyze the expression levels of elastase(NE) and miR-551 b in prostatitis massage fluid(EPS) of infertility men with prostatitis, and to study their diagnostic value. Methods: Between July 2017 and May 2019, 60 infertility men with prostatitis were selected in observation group, and 60 healthy male volunteers were selected in control group during the same period. The expressions levels of NE and miR-551 b in EPS of man were compared between the two groups. The correlation between NE level and miR-551 b level was analyzed. And the diagnostic efficacy and the area under ROC curve(AUC) of the levels of NE and miR-551 b of infertility men with prostatitis were also analyzed. Results: The NE expression level of men in the observation group was 1472.25(812.23, 1735.65), which was significant higher than that [156.38(76.35, 226.52)] of men in the control group. The expression level of mir-551 b of men in the observation group was 5.13(2.34, 13.47), which was significant lower than that [22.31(8.64, 42.23)] of men in the control group(P<0.05). Spearman rank correlation analysis showed that NE level was negative correlated with the miR-551 b level of men in the observation group(r=-0.874, P<0.05). The sensitivity, the specificity, and the accuracy of the levels of NE and miR-551 b for combined diagnosing prostatitis of infertility men were 79.2%, 81.5%, and 84.3%, respectively, which were all significant higher than those of diagnosing by NE level or miR-551 b level only. The AUC of NE and miR-551 b for combined diagnosing infertility of man with prostatitis was also significant higher than that of diagnosing by NE level or miR-551 b level only(P<0.05). Conclusion: The combined of NE and miR-551 b levels in EPS has better value for diagnosing infertility of men with prostatitis, which is expected to be molecular markers for diagnosing infertility of men with prostatitis.

关 键 词：前列腺炎 不育症 前列腺按摩液 弹性蛋白酶 miR-551b 诊断价值 

分 类 号：R697.33[医药卫生—泌尿科学] R698.2[医药卫生—外科学]