基于量子点的荧光共振能量转移探针的应用  

Application of fluorescence resonance energy transfer probes based on quantum dots

作　　者：李欣 刁娟娟 李莉[2] LI Xin;DIAO Juan-juan;LI Li(CentralLaboratory,Xinjiang Medical University,Urumqi 830011,China;School of Pharmacy,Xinjiang Medical University,Urumqi 830011,China)

机构地区：[1]新疆医科大学中心实验室,新疆乌鲁木齐830011 [2]新疆医科大学药学院,新疆乌鲁木齐830011

出　　处：《生物技术》2020年第3期295-301,311,共8页Biotechnology

基　　金：国家自然科学基金项目(“基于分子印迹传感器结合石墨烯量子点的异丙酚微流控芯片即时检测方法研究”,81760645)。

摘　　要：量子点因其独特的光学性质,以及可与有机分子所形成的偶联物的特殊性质,在光学生物标记,由其是荧光共振能量转移(Fluorescence resonance energy transfer,FRET)探针的合成与应用等领域具有广泛的应用前景,并因其实时、准确、灵敏的检测优势,在生物及医学领域始终被热切关注。该文以量子点的优势为基础,分别介绍了用于检测核酸、蛋白酶、生物反应及细胞状态的量子点-FRET探针的研究机理研究进展及应用优势。并对量子点-FRET探针的存在问题及研究方向进行了展望,为进一步进行该领域的研究提供理论支撑。Due to the unique optical properties and the special properties of conjugates formed with organic molecules,quantum dots have broad application prospects in the fields of optical biomarkers especially fluorescence resonance energy transfer probes.And because of its real-time,accurate and sensitive detection advantages,it has always been eagerly paid attention in the biological and medical fields.In this paper,the mechanism,progress and application advantages of quantum dot-FRET probes for detecting nucleic acids,proteases,biological reactions and cell states would be introduced.Meanwhile,the disadvantage and research directions of quantum dot-FRET probes are prospected,which provides theoretical support for further research in this field.

关 键 词：量子点 荧光共振能量转移 生物探针 生物检测 

分 类 号：Q503[生物学—生物化学]