基于Overview和物理图谱的大豆主茎节数候选基因挖掘  

Candidate Gene Mining of Soybean Node Numbers on the Main Stem Based on Overview and Physical Map of Soybean Genome

作　　者：尹振功[1,2] 王强[2] 孟宪欣[2] 刘广阳[2] 郭怡璠[2] 王秀君[2] 魏淑红[2] 来永才[1] YIN Zhen-gong;WANG Qiang;MENG Xian-xin;LIU Guang-yang;GUO Yi-fan;WANG Xiu-jun;WEI Shu-hong;LAI Yong-cai(Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme,Harbin 150086,China;Institute of Crop Resources,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China)

机构地区：[1]黑龙江省农业科学院博士后科研工作站,黑龙江哈尔滨150086 [2]黑龙江省农业科学院作物资源研究所,黑龙江哈尔滨150086

出　　处：《大豆科学》2020年第3期370-376,共7页Soybean Science

基　　金：黑龙江省农业科学院院级课题(2018YYYF021);七大农作物育种资助项目(2016YFD2100201-15);农业部农作物种质资源保护与利用专项(2017NWB036-23);科技部、财政部国家科技基础条件平台子平台资助项目(NICGR2017-024)。

摘　　要：利用与大豆主茎节数性状相关的54个原始QTL位点,应用Overview方法首次以大豆参考基因组物理图谱为背景进行整合和分析,得到11个重演性较好的置信区间,分布在大豆D1b、C2、B1、F、L和I连锁群上,其中L连锁群重演性较好的置信区间较多。对得到的候选区段进行基因注释得到488个候选基因,其中Glyma.11G087300、Glyma.20G014300、Glyma.13G221400、Glyma.06G243500、Glyma.13G052900、Glyma.13G052700参与植物激素信号转到通路(ID:Ko04075),推测这6个基因通过该通路的赤霉素途径和生长素途径参与大豆主茎节数的遗传调控。本研究所挖掘到的与茎生长发育及主茎节数直接相关的通路和候选基因能够为构建理想株型和大豆分子辅助育种提供新思路。This study integrated and analysisd 54 QTLs related to node numbers on main stem based on the physical map of soybean reference genome for the first time. 11 confidence intervals with good reproducibility were obtained by overview analysis on the D1 b, C2, B1, F, L and I linkage groups, and the L-linked group had more reciprocal confidence intervals. Gene annotation of the obtained candidate segments yielded 488 candidate genes, among which Glyma.11 G087300, Glyma.20 G014300, Glyma.13 G221400, Glyma.06 G243500, Glyma.13 G052900 and Glyma.13 G052700 participated in the plant hormone signal transduction(ID: Ko04075). It was speculated that these six genes play the genetic regulation role of soybean main stem through the gibberellin pathway and the auxin pathway. The pathways and candidate genes directly related to stem growth and number of main stem segments discovered in this study will provide new ideas for the construction of ideal plant type and soybean molecular assisted breeding.

关 键 词：大豆 主茎节数 QTL OVERVIEW 候选基因 

分 类 号：S565.1[农业科学—作物学]