过表达dnaJ基因对鲍曼不动杆菌生物膜形成的影响  

The effect of dnaJ overexpression on biofilm forming ability of Acinetobacter baumannii

作　　者：赖必鹏 周燕斌[1] 卓超[2] 刘鲍默 黄丽霞[1] 李少丽[1] 谷金萃 林子盈 汪昕 陈思民 杨锐富 Lai Bi-peng;Zhou Yan-bin;Zhuo Chao;Liu Bao-mo;Huang Li-xia;Li Shao-li;Gu Jin-cui;Lin Zi-ying;Wang Xin;Chen Si-min;Yang Rui-fu(Department of Pulmonary and Critical Care Medicine,the First Affiliated Hospital of Sun Yat-sen University,Guangzhou 510080;The First Affiliated Hospital of Guangzhou Medical University,Guangzhou Institute of Respiratory Health,Guangzhou 510120)

机构地区：[1]中山大学附属第一医院呼吸与危重症医学科,广州510080 [2]广州医科大学附属第一医院,广州呼吸健康研究院,广州510120

出　　处：《中国抗生素杂志》2020年第5期501-507,共7页Chinese Journal of Antibiotics

基　　金：国家自然科学基金(No.81570008)。

摘　　要：目的研究过表达dnaJ基因对鲍曼不动杆菌生物膜形成的影响。方法以ATCC19606基因组DNA为模板扩增dnaJ基因并与质粒pWH1266双酶切后连接,筛选重组质粒并转化菌株ATCC19606,从而构建dnaJ基因过表达菌株19606-p-dnaJ,类似的办法构建空质粒对照组19606-p,用半定量结晶紫染色法观察生物膜形成的差异。结果dnaJ基因过表达组dnaJ基因相对表达量显著高于空质粒对照组,dnaJ基因过表达组24h生物膜量显著低于空质粒对照组(P<0.001)。结论过表达dnaJ基因对鲍曼不动杆菌生物膜形成能力有显著抑制作用。Objective To investigate the effect of overexpressed dnaJ on biofilm-forming ability of Acinetobacter baumannii.Methods The dna J gene was amplified from ATCC19606 genome and cloned into plasmid pWH1266.The recombinant plasmid was then transformed into strain ATCC19606 to generate the recombinant strain 19606-p-dnaJ,in which dnaJ gene was overexpressed.At the same time,the control strain 19606-p bearing empty plasmid pWH1266 was constructed by a similar method.The difference of biofilm-forming ability was then observed through the crystal violet experiment.Results The dnaJ expression was significantly higher in recombinant strain 19606-p-dnaJ than that in control strain 19606-p.However,the amount of biofilm in recombinant strain 19606-p-dnaJ was significantly lower than that in control strain 19606-p(P<0.001).Conclusion The dnaJ overexpression can significantly inhibit the biofilm-forming ability of Acinetobacter baumannii.

关 键 词：鲍曼不动杆菌 dnaJ基因 过表达 生物膜 

分 类 号：R978.1[医药卫生—药品] R378[医药卫生—药学]