利用离体小孢子培养技术培育心里美萝卜新品系  

Development of Inbred Lines of Raphanus sativus L.var.‘Xinlimei’by Isolated Microspore Culture Technology

作　　者：张丽[1] 王庆彪[1] 王艳萍 ZHANG Li;WANG Qing-biao;WANG Yan-ping(Beijing Vegetable Research Center,Beijing Academy of Agriculture and Forestry Sciences,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China),Ministry of Agriculture and Rural Affairs,Beijing Key Laboratory of Vegetable Germplasm Improvement,Beijing 100097,China)

机构地区：[1]北京市农林科学院蔬菜研究中心,农业农村部华北地区园艺作物生物学与种质创制重点实验室,蔬菜种质改良北京市重点实验室,北京100097

出　　处：《中国蔬菜》2020年第8期53-56,共4页China Vegetables

基　　金：国家自然科学基金项目(31601753);北京市农林科学院创新能力建设项目(KJCX20170710,KJCX20170102)。

摘　　要：以北京心里美和山东心里美为供体材料进行离体小孢子培养,2个基因型均成功获得胚状体,每105个小孢子的成胚率分别为15.6%和21.3%。选取子叶形胚进行胚状体再生,北京心里美与山东心里美的成苗率分别为78.9%和64.5%。利用流式细胞仪对再生株倍性进行鉴定,44.8%为二倍体(双单倍体,DH系),17.2%为单倍体,38.1%为多倍体。选择正常的二倍体植株进行自交留种,并对自交后代进行田间性状鉴定,与供体植株相比,DH系出现较大的性状变异。对来源于北京心里美的肉质根圆形、绿皮血红肉和椭圆形、绿皮血红肉DH系进行扩繁,后代性状整齐一致,血红肉率100%,没有出现浅绿肉变异株。利用离体小孢子培养技术获得了肉色整齐一致的心里美萝卜新品系,有效解决了常规自交易出现浅绿肉变异株的问题。Taking‘Beijing Xinlimei’and‘Shandong Xinlimei’as donor materials,in vitro microspore culture was carried out.Embryoids were successfully obtained from both genotypes,and the embryo formative rate of each 105 microspore was 15.6%and 21.3%,respectively.Cytoledon-stage embryoes were chosen to conduct embryoid regenoration.The planting percents of‘Beijing Xinlimei’and‘Shandong Xinlimei’were 78.9%and 64.5%,respectively.Flow cytometry was used to identify ploidy of regenerated plantlets.44.8%of them were diploid(double haploid,DH line),17.2%were haploid and 38.1%were polyploid.Normal DH lines were selected to conduct self-crossing and reserve seed for planting.And field traits identification were carried out on their progenies.The DH lines exhibited bigger variation compared with the donor plants.The DH lines derived from‘Beijing Xinlimei’with round fleshy root,green peel,blood red pulp,and oval green peel,blood red pulp were propagated and their progenies characters were uniform.The rate of blood red pulp reached 100%,and there were no variant plants with light green pulp.New‘Xinlimei’lines with uniformed pulp color were obtained by in vitro microspore culture technology.This had effectively solved the problem occurred easily during conventional selfing of mutant plants with light green pulp.

关 键 词：萝卜 离体小孢子培养 再生 品系 

分 类 号：S631.1[农业科学—蔬菜学]