一株O型口蹄疫病毒在C57BL/6小鼠和原代细胞的传代及其全序的测定和分析  

Passage of a type O foot-and-mouth disease virus in mice and cell and analysis of its complete sequence

作　　者：龚婷[2] 黄书伦 查晶晶 孙普[1] 马雪青[1] 白兴文[1] 袁红[1] 曹轶梅[1] 李坤[1] 张婧[1] 卢曾军[1] 刘在新[1] 李平花[1] Ting Gong;Shulun Huang;Jingjing Zha;Pu Sun;Xueqing Ma;Xingwen Bai;Hong Yuan;Yimei Cao;Kun Li;Jing Zhang;Zengjun Lu;Zaixin Liu;Pinghua Li(State Key Laboratory of Veterinary Etiological Biology,National Foot-and-Mouth Disease Reference Laboratory,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,Gansu Province,China;Henan University of Animal Husbandry and Economy,Zhengzhou 450000,Henan Province,China)

机构地区：[1]中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室,国家口蹄疫参考实验室,甘肃兰州730046 [2]河南牧业经济学院,河南郑州450000

出　　处：《微生物学报》2020年第7期1413-1420,共8页Acta Microbiologica Sinica

基　　金：“十三五”国家重点研发计划(2017YFD0501104)。

摘　　要：【目的】旨在成功建立FMDVC57BL/6小鼠的实验感染模型。【方法】采用体内和体外循环适应传代的方法,选取一株对C57BL/6小鼠不敏感FMDVO/HK/CHA/99MF4,将其在C57BL/6小鼠(体内)和胎猪肾原代细胞FPK(体外)进行多次循环适应传代。【结果】成功获得一株对C57BL/6小鼠敏感的FMDVO/HK/CHA/99MF4C5株。【结论】本研究成功建立了FMDV突变株感染C57BL/6小鼠的实验动物模型,为未来FMD疫苗效力的评估和致病性相关的研究奠定了基础。[Objective]To successfully establish the C57 BL/6 mice experimental infection model for foot-and-mouth disease virus.[Methods]The foot-and-mouth disease virus O/HK/CHA/99 MF4 strain,which is insensitive to C57 BL/6 mice,was selected and continuously passaged in C57 BL/6 mice(in vivo)and fetal pig kidney cells(in vitro).[Results]We successfully obtained a foot-and-mouth disease virus mutant(O/HK/CHA/99 MF4 C5),which was sensitive to C57 BL/6 mouse.[Conclusion]This study successfully established C57 BL/6 mice model for a foot-and-mouth disease virus mutant strain,which lays a foundation for the evaluation of the efficacy of foot-and-mouth disease vaccine and the study on pathogenicity in the future.

关 键 词：口蹄疫病毒 小鼠 细胞 循环传代 全序的测定与分析 

分 类 号：S852.65[农业科学—基础兽医学]