“三棱-莪术”组分配伍对子宫肌瘤大鼠下丘脑和垂体激素的影响  

The effect of Curcumae Rhizoma and Sparganii Rhizoma component compatibility on hormones in the hypothalamic and pituitary of uterine leiomyoma rats

作　　者：付煜 徐秋霞 张黎 赵玉民 刘捷 欧凯西 王刚 彭腾[1] 王勇 丁维俊[1] 余成浩[1] FU Yu;XU Qiu-xia;ZHANG Ni;ZHAO Yu-ming;LIU Jie;OU Kai-xi;WANG Gang;PENG Teng;WANG Yong;DING Wei-jun;YU Cheng-hao(Chengdu University of Traditional Chinese Medicine,Chengdu 610075,Sichuan;Shenzhen Hospital of the University of Chinese Academy of Sciences,Shenzhen 518106,Guangdong;Sichuan University,Chengdu 610065,Sichuan)

机构地区：[1]成都中医药大学基础医学院,四川成都610075 [2]中国科学院大学深圳医院,广东深圳518106 [3]四川大学生物材料工程研究中心,四川成都610065

出　　处：《中药与临床》2020年第2期31-36,共6页Pharmacy and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金(NSFC):81001668,81673878;四川省青年科技创新研究团队项目:2020JDTD0022;中医药“杏林学者”学科人才研究促进计划:XSGG2019006。

摘　　要：目的:研究"三棱-莪术"组分配伍对子宫肌瘤大鼠下丘脑和垂体相关激素的影响。方法:将未生孕的SD雌性大鼠随机分为6组:正常对照组(NC),模型组(UL),桂枝茯苓胶囊组(GZFLC),"三棱-莪术"组分配伍高剂量组(HCRSR)、中剂量组(MCRSR)及低剂量组(LCRSR)。经肌注孕酮和灌胃己烯雌酚来诱导建立子宫肌瘤大鼠模型。经过五周的造模和药物治疗后,收集大鼠股动脉血、下丘脑及垂体,通过ELISA法测定血清,下丘脑和垂体中促性腺激素释放激素(GnRH),促卵泡激素(FSH)和促黄体激素(LH)的水平。结果:该造模方法成功地建立了大鼠子宫肌瘤模型。各组血清中GnRH,FSH和LH的浓度,以及下丘脑中的GnRH水平均无统计学意义(P> 0.05)。模型组垂体中FSH和LH的表达高于正常组(P <0.01)。治疗后,高剂量(HCRSR)和桂枝茯苓胶囊组(GZFLC)组中"三棱-莪术"组分配伍的FSH和LH水平显著降低(P <0.01)。结论:这些数据表明"三棱-莪术"组分配伍可以降低子宫平滑肌瘤大鼠垂体中FSH和LH的表达。Objective:To investigate the effect of the Curcumae Rhizoma and Sparganii Rhizoma (CRSR) component compatibility on Hormones in the Hypothalamic and Pituitary of the Uterine Leiomyoma (UL) rats model.Method:Non-pregnant female Sprague-Dawley rats were randomly divided into six groups:normal control (NC)group,UL model (UL)group,GuiZhiFuLing Capsule (GZFLC)group,Curcumae Rhizoma and Sparganii Rhizoma therapy(CRSR) group,including high-dose CRSR therapy (HCRSR) group,middle-dose CRSR therapy (MCRSR) group and low-dose CRSR therapy(LCRSR) group.The UL rat model was induced by intramuscular injection of progesterone and intragastric gavage of diethylstilbestrol.For 5 weeks of model-made and drug treatment,ELISA was used to assay the levels of GnRH,FSH,and LH in serum,hypothalamic and pituitary.Result:The UL model in rats was established successfully.The GnRH,FSH and LH concentrations in serum of all groups,including the GnRH level in the hypothalamic,have no statistical difference (P>0.05).However,we found that the expression of FSH and LH in the pituitary in the UL group was higher than in NC groups(P<0.01).After treatment,the levels of FSH and LH was significantly decreased in the HCRSR and GZFLC groups (P<0.01).Conclusion:These data suggest that the effect of CRSR can reduce the expression of FSH and LH in the pituitary of Uterine Leiomyoma Rats.

关 键 词：子宫肌瘤 三棱总黄酮 莪术挥发油 组分配伍 促性腺激素释放激素 促卵泡激素 促黄体 

分 类 号：R285.5[医药卫生—中药学]