大白菜小植株突变体s1光合能力及光反应相关基因的转录组分析  

Photosynthetic capacity and transcriptome analysis of genes related to light reaction of small plant mutant s1 in Chinese cabbage

作　　者：王倩云 卢银[2] 王彦华[2] 赵建军[2] 申书兴[2] 冯大领[1,2] WANG Qianyun;LU Yin;WANG Yanhua;ZHAO Jianjun;SHEN Shuxing;FENG Daling(College of Life Sciences,Hebei Agricultural University,Baoding 071001,China;State Key Laboratory of North China Crop Improvement and Regulation/Key Laboratory for Vegetable Germplasm Innovation and Utilization of Hebei,Hebei Agricultural University,Baoding 071001,China)

机构地区：[1]河北农业大学生命科学学院,河北保定071001 [2]河北农业大学省部共建华北作物改良与调控国家重点实验室/河北省蔬菜种质创新与利用重点实验室,河北保定071001

出　　处：《河北农业大学学报》2020年第3期50-54,67,共6页Journal of Hebei Agricultural University

基　　金：国家自然科学基金项目(31872100,31672151,31572120);河北省科技计划项目(16226304D-2);河北省自然科学基金项目(C2018204135);河北省高等学校科学技术研究项目(BJ2019020).

摘　　要：植株的大小与光合能力密切相关。本研究以大白菜小植株突变体s1和其野生型WT为材料,通过测定叶绿素含量、净光合速率、叶绿素荧光参数以及对光反应相关基因的转录水平分析,解析大白菜植株大小与光合能力的相关性。结果表明:与野生型相比,大白菜小植株突变体s1的叶绿素含量无显著差异,但光合速率显著降低29%;Fv/Fm和Y(II)值在二者之间无显著差异(P>0.05),而s1的外叶和新叶ETR值都显著低于WT;进一步的转录组分析表明光合电子传递链上的PSII、PC、FNR、ATP合酶相关基因表达下调0.94~1.69倍。由此证实,大白菜小植株突变体s1光合速率低与光合电子传递链中的基因表达下降及光合电子传递速率下降有关。该研究为进一步挖掘大白菜植株大小相关的优异基因资源提供了良好的试验材料,也为大白菜分子设计育种提供了理论依据。Plant size is closely related to photosynthetic capacity.In this study,small plant mutant s1 and its wildtype were used as materials.The correlation between plant size and photosynthetic capacity of Chinese cabbage was analyzed through the determination of chlorophyll content,net photosynthetic rate,fluorescence parameters and transcriptional level analysis of genes related to light reaction.The results showed that there was no significant difference in chlorophyll content between mutant s1 and its wild type(WT),but the photosynthetic rate of s1 was significantly lower than that of WT.There was no significant difference in Fv/Fm and Y(II)values between s1 and WT,while the ETR values of outer and new leaves of s1 were significantly lower than that of WT.RNA-seq analysis confirmed that genes related to PSII,PC,FNR and ATP synthase on photosynthetic electron transport chain were down-regulated by 0.94-1.69.It was confirmed that the low photosynthetic rate of small plant mutant s1 of Chinese cabbage was related to the decrease of gene expression and photosynthetic electron transport rate in photosynthetic electron transport chain.This research provides a good experimental material for further exploring the excellent gene resources related to plant size of Chinese cabbage,and also provides a theoretical basis for molecular design breeding of Chinese cabbage.

关 键 词：大白菜 小植株突变体 光合速率 叶绿素荧光参数 光合电子传递 

分 类 号：S634.3[农业科学—蔬菜学]