拟南芥T-DNA插入株系SALK_037453叶发育突变位点的鉴定和分析  

Identification and analysis of leaf development mutation sites in Arabidopsis T-DNA insertion line SALK_037453

作　　者：司旭阳 李文静[1] 张洪艳 张科[1] 潘延云[1] SI Xuyang;LI Wenjing;ZHANG Hongyan;ZHANG Ke;PAN Yanyun(Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology,Hebei Agricultural University,Baoding 071000,China)

机构地区：[1]河北农业大学河北省植物生理与分子病理学重点实验室,河北保定071000

出　　处：《河北农业大学学报》2020年第3期55-60,共6页Journal of Hebei Agricultural University

基　　金：河北省自然科学基金项目(C2017204095);河北省高等学校科学技术研究项目(ZD2017039).

摘　　要：对来自Salk库的拟南芥T-DNA插入突变体SALK037453的表型分析发现,该突变体株系因莲座叶叶柄较短而呈现聚集表型。遗传分析和PCR检测显示,SALK037453突变体的表型与网站提供的T-DNA插入位点所在基因不连锁,应是由另外的未知插入位点的T-DNA引起的。为了寻找该位点,鉴定导致莲座叶聚集表型的基因,本研究利用TAIL-PCR的方法,鉴定出与突变表型连锁的T-DNA插入位点,位于基因At4G39400(AtBRI1)和At4G39403(AtPLS)之间。进一步利用qRT-PCR的方法检测,发现该位点由于T-DNA的插入,同时影响了AtBRI1和AtPLS的转录水平的表达。该研究为探索叶发育的过程提供了新的候选基因,并为进一步阐明AtBRI1和AtPLS的生物学功能提供了新材料。An Arabidopsis T-DNA insertion mutant SALK037453 from the Salk library showed short petioles and defective leaf development.The genetic analysis and PCR detection showed that the phenotype of the Salk037453 mutant was not linked to the insertion site provided by the website and should be caused by another T-DNA at an unknown insertion site.In order to find this site and identify the genes that cause the rosette leaf aggregation phenotype,TAIL-PCR was used to identify the sites and it was found that the T-DNA located between the two genes,At4 G39400(At BRI1)and At4 G39403(At PLS).Furthermore,real-time PCR results showed that transcription level of the both genes had changed due to the inserted T-DNA.This study provides new candidate genes for exploring the process of leaf development and provides new materials for further elucidating the biological functions of At BRI1 and At PLS.

关 键 词：SALK037453 TAIL-PCR 叶发育 基因表达 

分 类 号：Q943.2[生物学—植物学]