盐渍环境微生物矿化碳酸钙技术可行性研究  

Feasibility study on microbial mineralization of calcium carbonate in saline environment

作　　者：杨晓旭 张文 赵媛[4] 张卫红 王珑霖 薛玮玮 YANG Xiaoxu;ZHANG Wen;ZHAO Yuan;ZHANG Weihong;WANG Longlin;XUE Weiwei(Department of Geological Engineering,Qinghai University,Xining 810016,China;College of Civil Engineering,Qinghai University,Xining 810016,China;Key Laboratory of Cenozoic Resources and Environment in the Northern Margin of Qinghai-Tibet Plateau,Xining 810016,China;College of Ecological Engineering,Qinghai University,Xining 810016,China;The Institute of Electric Power Design of Qinghai,Xining 810086,China)

机构地区：[1]青海大学地质工程系,西宁810016 [2]青海大学土木工程学院,西宁810016 [3]青藏高原北缘新生代资源环境重点实验室,西宁810016 [4]青海大学生态工程学院,西宁810016 [5]青海省电力设计院,西宁810086

出　　处：《黑龙江畜牧兽医》2020年第13期26-31,163,共7页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(51468055);青海省级重点实验室建设项目(2017-ZJ-Y30);青海省“135”人才工程项目。

摘　　要：为了从青海省寒旱环境的盐渍土、水、植物中筛选分离及鉴定可以生产脲酶的耐盐菌,改善青海牧区土壤环境,探索寒旱盐渍土环境中微生物矿化碳酸钙技术的可行性,试验利用5%盐浓度的筛选培养基筛选耐盐菌,从中筛选最优脲酶产生菌,测定其生长曲线和产酶曲线,并进行菌株鉴定及微生物矿化碳酸钙技术可行性测定。结果表明:筛选出200株耐盐菌,其中分离得到1株高产耐盐脲酶产生菌,该菌株在5%盐浓度下的液体培养基中于37℃、180 r/min振荡培养时,其对数生长期出现在4~24 h,最佳发酵时间为48 h,初始酶活性最高达到2.21 U/mL,经鉴定该菌株为木糖葡萄球菌,登录号为MN420830,生成的沉淀为碳酸钙。说明从青海省寒旱盐渍土环境中可以筛选出耐盐脲酶产生菌,且该菌在实验室环境条件下进行微生物矿化碳酸钙技术是可行的。In order to screen and isolate the salt-tolerant bacteria that can produce urease from the saline soil,water and plants in the cold and drought environment of Qinghai province,improve the soil environment in pastoral areas of Qinghai province,and explore the feasibility of microbial mineralized calcium carbonate technology in the cold and drought saline soil environment.In this study,the salt-tolerant bacteria were screened by a screening medium with 5%salt concentration,the optimal urease-producing bacteria were screened,the growth curve and enzyme production curve were determined,and the strain identification and the feasibility of microbial mineralized calcium carbonate were determined.The results showed that 200 strains of salt-tolerant bacteria were screened,and 1 high-yield salt-tolerant urease-producing strain was isolated.The strain was cultured in a liquid medium at 5%salt concentration at 37℃and 180 r/min.The logarithmic growth period was 4-24 h,the optimal fermentation time was 48 h,and the initial enzyme activity was up to 2.21 U/mL.The strain was identified as Staphylococcus aureus with accession number MN420830,and the precipitate formed was calcium carbonate.It was indicated that the salt-tolerant urease-producing bacteria could be screened out from the cold and drought saline soil environment in Qinghai province,and the microbial mineralization of calcium carbonate was feasible in the laboratory.

关 键 词：盐渍土环境 耐盐微生物 脲酶产生菌 酶活性 微生物矿化碳酸钙技术 X射线衍射 

分 类 号：Q93-335[生物学—微生物学]