细胞块免疫化学染色在胸腹腔积液病理学诊断中的应用  

The Application of Cell Block Immunochemical Staining in the Pathological Diagnosis of Pleural and Peritoneal Effusion

作　　者：季月霞 丘佳明[2] 仲玉英 郭天威 徐逸 沈轶凡 罗燕 张玉兰 JI Yue-xia;QIU Jia-ming;ZHONG Yu-ying;GUO Tian-wei;XU Yi;SHEN Yi-fan;LUO Yan;ZHANG Yu-lan(Changshu Traditional Chinese Medicine Hospital,Changshu New Area Hospital,Changshu,Jiangsu,215500,China;Changshu No.2 People’s Hospital,Changshu,Jiangsu,215500,China)

机构地区：[1]常熟市中医院,常熟市新区医院,江苏常熟215500 [2]常熟市第二人民医院,江苏常熟215500

出　　处：《中国血液流变学杂志》2019年第4期483-486,共4页Chinese Journal of Hemorheology

摘　　要：目的探讨细胞块免疫化学染色在胸腹腔积液病理学诊断中的准确性.方法收集常熟市中医院病理科具有疑似或高度疑似恶性胸腹腔积液的患者共81例,采用三种不同方法:常规细胞涂片HE染色,细胞块HE染色和细胞块免疫化学染色分别进行观察,比较其对临床诊断定性的可靠性.结果采用细胞块切片方法阳性诊断率高于常规细胞涂片;细胞块切片免疫化学染色阳性诊断率高于前两组,且不确定性明显减少,差异有统计学意义(P<0.05).综合诊断的阳性率要明显高于常规细胞染色和细胞块HE染色,不确定率远低于后两种方法,差异有统计学意义(P<0.05).细胞块免疫化学染色与综合诊断比较,诊断结果非常接近,差异无统计学意义(P>0.05).结论运用细胞块免疫化学染色在提高胸腹腔积液细胞病理学诊断的准确性中值得推广应用.Objective To investigate the accuracy of cell block immunochemical staining in the pathological diagnosis of pleural and peritoneal effusion.Methods 81 patients with suspected or highly suspected malignant pleural or peritoneal effusion were collected from the pathology department of Changshu Traditional Chinese Medicine Hospital.Three different methods were used to observe and compare their reliability in clinical diagnosis,including routine cell smear HE staining,cell block HE staining and cell block immunochemical staining.Results The positive diagnosis rate of cell block section was higher than that of conventional cell smear,and the positive diagnosis rate of immunochemical staining of cell block section was higher than that of the former two groups,and the uncertainty was significantly reduced,the difference was statistically significant(P<0.05).The positive rate of comprehensive diagnosis was significantly higher than that of routine cell staining and cell block HE staining,and the uncertainty rate was much lower than that of the latter two methods(P<0.05).Compared with the comprehensive diagnosis,the result of immunocytochemical staining was very close,and the difference was not statistically significant(P>0.05).Conclusion The application of cell block immunochemical staining in the diagnosis of pleural and peritoneal effusion is worth popularizing.

关 键 词：免疫化学染色 胸腹腔积液 细胞块 细胞学 HE染色 

分 类 号：R446.8[医药卫生—诊断学] R730.4[医药卫生—临床医学]