脂肪组织冻存的方法研究  

Study on the Method of Cryopreservation of Adipose Tissue

作　　者：夏洁芳 徐峰波 李雁冰 田雨 王战涛 张建中[3] XIA Jie-fang;XU Feng-bo;LI Yan-bing;TIAN Yu;WANG Zhan-tao;ZHANG Jian-zhong(Shaanxi Stem Cell Engineering Co.,Ltd.,Xi'an 710000,Shaanxi,China;Yinfeng Bioengineering Group Co.,Ltd.,Jinan 250000,Shandong,China;Department of Endocrinology,China-Japan Lianyi Hospital of Jilin University,Changchun 130033,Jilin,China)

机构地区：[1]陕西干细胞工程有限公司,陕西西安710000 [2]银丰生物工程集团有限公司,山东济南250000 [3]吉林大学中日联谊医院内分泌科,吉林长春130033

出　　处：《医学信息》2020年第14期24-26,共3页Journal of Medical Information

基　　金：吉林省科技发展计划项目(编号:20180201029YY)。

摘　　要：自体脂肪组织因具有来源丰富、易于取材、操作简单,无排异反应等优点,使得自体脂肪移植成为医疗美容行业进行面部凹陷填充、丰胸丰臀等整形手术时较为流行的技术。手术为达到较高的移植存活率和整形效果,需要对患者进行多次的脂肪抽吸并重复移植,过程较长,增加患者痛苦。因此,一次性抽吸足够量的脂肪组织进行冻存,再次移植时复苏使用便成为了解决这一问题的最优方法。本文现就脂肪组织的采集、取脂纯化方法、冻存保护剂的选择、冻存复苏方法及提高移植存活率研究做一综述。Autologous fat tissue has the advantages of abundant sources,easy access to materials,simple operation,and no rejection reaction.Autologous fat transplantation has become a popular technique for plastic surgery in the medical and cosmetic industry for facial depression filling,breast enlargement and hip buttocks.In order to achieve a high transplant survival rate and plastic effect,it is necessary to perform multiple liposuctions and repeat transplantation on the patient.The process is longer and increases the patient's pain.Therefore,suctioning a sufficient amount of adipose tissue for cryopreservation at a time,and resuscitating it for transplantation again become the best way to solve this problem.This article will review the research on the collection of adipose tissue,the method of purifying fat,the choice of cryoprotectant,the method of cryopreservation and resuscitation,and the improvement of transplant survival rate.

关 键 词：脂肪组织 取脂方法 冻存保护剂 冻融 移植成活率 

分 类 号：R622.9[医药卫生—整形外科]