DNA甲基转移酶3A调控少突胶质细胞发育  

The Regulation Role of DNMT3A in the Development of Oligodendrocytes

作　　者：方敏溪 俞倩 易敏[1] 杨爱芬[1] 徐晓锋 FANG Minxi;YU Qian;YI Min;YANG Aifen;XU Xiaofeng(Zhejiang Key Laboratory of Organ Development and Regeneration,Institute of Life Sciences,Hangzhou Normal University,Hangzhou 311121,China)

机构地区：[1]杭州师范大学生命科学研究院,浙江省器官发育与再生技术研究重点实验室,浙江杭州311121

出　　处：《杭州师范大学学报（自然科学版）》2020年第4期376-381,共6页Journal of Hangzhou Normal University(Natural Science Edition)

基　　金：浙江省自然科学基金项目(LQ17C040001,LQ16C090004).

摘　　要：包括DNA甲基化在内的表观遗传修饰参与调控少突胶质细胞的发育.DNA甲基转移酶3A(DNMT3A)负责催化DNA的从头甲基化,参与调控多种器官的发育.为了确定DNMT3A是否参与少突胶质细胞的发育,本研究利用Dnmt3a全局性敲除小鼠,通过RNA原位杂交检测少突胶质细胞的发育.结果发现,缺失DNMT3A的小鼠体内少突胶质细胞的分化被延迟,少突胶质前体细胞增多,说明DNMT3A的功能对于少突胶质细胞的正常发育是必需的.Epigenetic modification including DNA methylation is involved in the regulation of oligodendrocyte(OL)development.DNMT3A is responsible for catalyzing de novo methylation of DNA and is involved in regulating the development of multiple organs.To determine its role in OL development,the paper uses Dnmt3a global knockout miceto detect OL development by RNA in situ hybridization.The results show that the loss of DNMT3A delays the differentiation of OLs and increases the number of oligodendrocyte precursor cells,which indicate that DNMT3A is essential for the normal development of OLs.

关 键 词：DNA甲基化 DNA甲基转移酶3A 少突胶质细胞 

分 类 号：Q189[生物学—神经生物学]