洗四方袋泡剂质量标准研究  

Quality Standard of Xisifang Bag Bubble

作　　者：黄振忠[1] 袁经权[2,3] 唐炳兰[2] 潘宇 周阳晓 陈路[2] HUANG Zhenzhong;YUAN Jingquan;TANG Binglan;PAN Yu;ZHOU Yangxiao;CHEN Lu(Combination of TCM and Western Medicine Orthopaedic Hospital of Guigang,Guigang,Guangxi,China 537100;Guangxi Botanical Garden of Madicinal Plants,Nanning,Guangxi,China 530023;Guangxi University of Chinese Medicine,Nanning,Guangxi,China 530001)

机构地区：[1]广西壮族自治区贵港市中西医结合骨科医院,广西贵港537100 [2]广西壮族自治区药用植物园,广西南宁530023 [3]广西中医药大学,广西南宁530001

出　　处：《中国药业》2020年第15期64-67,共4页China Pharmaceuticals

基　　金：广西壮族自治区贵港市科技计划项目[贵科攻1305004]。

摘　　要：目的建立洗四方袋泡剂的质量标准。方法采用薄层色谱(TLC)法定性鉴别制剂中鸡血藤、三叉苦和大黄药材;采用高效液相色谱法测定制剂中大黄酸的含量,色谱柱为Agilent C18柱(250 mm×4.6 mm,5μm),流动相为甲醇-0.1%磷酸溶液(80∶20,V/V),柱温为30℃,流速为1.0 m L/min,检测波长为254 nm,进样量为10μL。结果鸡血藤、三叉苦和大黄的薄层色谱图斑点清晰,分离度较好,阴性对照无干扰;大黄酸质量浓度线性范围为0.044~0.352μg(R2=0.9997),平均回收率为98.13%,RSD为1.77%(n=9)。结论该方法可靠,准确,操作简单,可用于洗四方袋泡剂的质量控制。Objective To establish the quality standard of Xisifang Bag Bubble.Methods A thin layer chromatography(TLC)method was used to identify Spatholobi caulis,Evodiae lepta and Rhei Radix et Rhizoma in the preparation.A high performance liquid chromatography(HPLC)method was applied to determine the content of rhein.The Agilent C18 column(250 mm×4.6 mm,5μm)was adopted,the mobile phase was methanol-0.1%phosphoric acid solution(80∶20,V/V),the flow rate was 1.0 m L/min,the column temperature was 30℃,the detecting wavelength was 254 nm,and the sample size was 10μL.Results The TLC plots of Spatholobi caulis,Evodiae lepta and Rhei Radix et Rhizoma showed clearly with well separation and no interference with the negative.Rhein showed good linear relationship in the range of 0.044-0.352μg(R2=0.9997)with the peak area.The average recovery was 98.13%and RSD was 1.77%(n=9).Conclusion This methods is reliable,accurate,and simple in operation,which can be used for the quality control of Xisifang Bag Bubble.

关 键 词：洗四方袋泡剂 质量标准 薄层色谱法 大黄酸 高效液相色谱法 

分 类 号：R932[医药卫生—生药学] R284.1[医药卫生—药学]