距离-温度联合的新型筛选法提高人精子质量的效果分析  

Effect analysis of improving human sperm quality by a novel distance-temperature-combined selection approach

作　　者：饶金鹏 邱枫[1] 蔡益婷[1] 魏凯[1] 金敏[1] 金帆[2] RAO Jin-peng;QIU Feng;CAI Yi-ting;WEI Kai;JIN Min;JIN Fan(Reproductive Medical Center, the Second Affiliated Hospital;Key Laboratory of Reproductive Genetics,Ministry of Education, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310000, China)

机构地区：[1]浙江大学医学院附属第二医院生殖医学中心 [2]浙江大学医学院附属妇产科医院生殖遗传教育部重点实验室,浙江杭州310000

出　　处：《基础医学与临床》2020年第8期1025-1030,共6页Basic and Clinical Medicine

基　　金：国家自然科学基金(81601268);浙江省医药卫生科技计划(2019PY028)。

摘　　要：目的探讨基于精子前向运动性和温度趋向性的新型筛选方法在提高人精子质量的效果。方法将密度梯度离心后获能的精子置于不同长度(2、5和8 cm)条状液滴的一端,同时设置不同的环境温度条件(恒温32℃、恒温37℃、32℃~37℃温度梯度),孵育60 min后,从液滴的另一端收集精子样本,比较不同距离及温度条件下筛选获得精子的浓度、活力、正常形态率及DNA碎片率等指标的差异。结果在相同的环境温度条件下,经过长距离(5、8 cm)筛选获得的精子比初始精子(0 cm)具有更高的正常形态率(P<0.05);随着距离的延长,精子活力未见显著性改变,但精子的浓度则呈极显著下降趋势(P<0.01)。长距离(5、8 cm)条件下,32℃~37℃温度梯度环境获得的精子浓度高于无温度差值组(恒温32℃和恒温37℃)(P<0.01),且精子DNA碎片率更低(P<0.05)。结论长距离水平游动能淘汰大量形态异常的精子,但过长的距离将导致精子浓度急剧下降,温度梯度的设置能富集得到更多DNA完整性较好的具有正常趋热应答能力的精子。5 cm,32℃~37℃条件下优选精子效果较好,可降低体外受精过程中缺陷精子与卵子发生结合的风险。Objective To investigate the effect of improving human sperm quality by novel selection approach based on progressive motility and thermotaxis.Methods The capacitated spermatozoa were placed on one end of strip droplets with different length(2,5,8 cm)and different temperature conditions(32℃,37℃or 32℃~37℃)after density gradient centrifugation.The spermatozoa were collected from the other end of the droplets after swimming for 60 min.The concentration,motility rate,normal morphology rate and DNA fragment rate of sperm were compared among different distance-temperature-combined selection conditions.Results Under the same tempera-ture condition,the spermatozoa selected by long distance(5,8 cm)had higher rate of normal morphology picture than the initial one(P<0.05).With the increase of distance,the sperm motility rate increased slightly,while the concentration of sperm decreased significantly(P<0.01).The sperm concentration in temperature gradient(32℃~37℃)was higher than that in no-gradient control groups(32℃and 37℃,P<0.01),while the spermDNA fragment rate was lower(P<0.05).Conclusions Through long distance swimming,a tremendous amount of sperm with abnormal morphology were eliminated,but long distance selection would also lead to a sharp drop in sperm concentration.The temperature gradients enrich more sperm with better DNA integrity and normal thermotaxis.Under the condition of 5 cm,32℃~37℃,the effect of sperm selection is better,which may reduce the risk of genetic defective sperm combining with oocytes during IVF.

关 键 词：精子 游动距离 温度趋向性 正常形态 DNA碎片 

分 类 号：R34[医药卫生—基础医学]