miR-10b对牛卵巢颗粒细胞凋亡的影响  

Effects of miR-10b on Apoptosis of Bovine Ovarian Granulosa Cells

作　　者：郭乐薇 赵静[1,2] 刘红羽[1,2] 王军[1,2] 吕文发[1,2] GUO Lewei;ZHAO Jing;LIU Hongyu;WANG Jun;LYU Wenfa(Joint Laboratory of Modern Agricultural Technology International Cooperation,Ministry of Education,Jilin Agricultural University,Jilin Changchun 130118,China;Key Lab of Animal Production,Product Quality and Security,Ministry of Education,Jilin Agricultural University,Jilin Changchun 130118,China)

机构地区：[1]吉林农业大学现代农业技术教育部国际合作联合重点实验室,吉林长春130118 [2]吉林农业大学动物生产与产品质量安全教育部重点实验室,吉林长春130118

出　　处：《中国畜牧杂志》2020年第7期115-119,共5页Chinese Journal of Animal Science

基　　金：国家自然科学基金(31772600)。

摘　　要：颗粒细胞是卵巢中必需的体细胞,在卵泡发育过程中起重要作用。为探究miR-10b对牛卵巢颗粒细胞凋亡的影响,本实验中2个处理组分别转染miR-10b模拟物(miR-10b mimics)和miRNA模拟物(mimics NC),转染48h后,通过Quantitativereal-timePCR(qRT-PCR)检测内源miR-10b表达量,明确miR-10b mimics转染成功。利用qRT-PCR和Western Blot检测凋亡相关蛋白Caspase-3、Bcl-2和Bax表达量的变化;利用流式细胞术检测颗粒细胞凋亡率。结果显示:与mimics NC组相比,miR-10b mimics组Bcl-2 mRNA和蛋白表达水平显著降低,Bax和Caspase-3 mRNA和蛋白表达水平显著升高;同时miR-10b mimics组的细胞凋亡率较mimics NC组显著升高。结果表明,miR-10b可促进牛卵巢颗粒细胞凋亡。Granulosa cell is an essential somatic cell in ovary and plays an important role in follicular development.The present study was performed to investigate the effects of miR-10b on apoptosis of bovine ovarian granulosa cell.Bovine ovarian granulosa cell was transfected with miR-10b mimics and mimics NC,respectively.After transfection for 48 h,the expression of endogenous miR-10b was detected by Quantitative real-time PCR(qRT-PCR)to confirm the successful transfection of miR-10b mimics.The expression levels of apoptosis-related proteins Caspase-3,Bcl-2 and Bax were detected by qRT-PCR and Western Blot;and granulosa cell apoptosis rate was detected by flow cytometry.The results showed that forced expression miR-10b significantly decreased Bcl-2 expression at both the mRNA and protein levels(P<0.05),while the mRNA and protein levels of Bax and caspase-3 were significantly increased(P<0.05)in granulosa cells.Furthermore,the apoptosis rate in miR-10b mimics group was significantly higher than that in mimics NC group(P<0.05).These results indicated that miR-10b could promote the apoptosis of bovine ovarian granulosa cells.

关 键 词：MIR-10B 卵巢颗粒细胞 凋亡 牛 

分 类 号：S823.2[农业科学—畜牧学]