人参皂苷F2干预Caspase级联反应抑制H2O2诱导的细胞凋亡  

Ginsenoside F2 Interferes with the Cascade Reactions of Caspase to Inhibit HO-induced Apoptosis

作　　者：刘迪 张浩 张寒雪 孙宏宇 郑美玉 冯宪敏 LIU Di;ZHANG Hao;ZHANG Han-xue;SUN Hong-yu;ZHENG Mei-yu;FENG Xian-min(Academy of Basic Medicine,Jilin Medical University,Jilin 132013,China;Academy of Medical Technology,Beihua University,Jilin 132013,China)

机构地区：[1]吉林医药学院基础医学院,吉林吉林132013 [2]北华大学医学技术学院,吉林吉林132013

出　　处：《现代食品科技》2020年第7期11-16,共6页Modern Food Science and Technology

基　　金：吉林省科技发展计划项目(20190103148JH);吉林省卫生健康科技能力提升计划项目(2019Q031);吉林省中医药科技项目(2019139)。

摘　　要：本研究探讨了人参皂苷F2对H2O2诱导人胚肾HEK-293细胞损伤的抗凋亡作用。利用试剂盒测定凋亡细胞Caspase-6和Caspase-9活性水平,应用WesternBlot检测凋亡细胞中Bcl-2凋亡家族(Bcl-2和Bax)与Caspase凋亡家族(CleavedCaspase-3和Cleaved PARP)的蛋白表达量。H2O2损伤细胞后,Caspase酶活力提高,促凋亡蛋白表达提高;1.25、5、20μmol/L F2预处理损伤细胞后,Caspase-6和Caspase-9活力呈浓度依赖性降低(p<0.05),当F2浓度达到20μmol/L时,Caspase-6活性降低了6.83 U/mg protein,Caspase-9活性降低了5.88U/mgprotein;CleavedCaspase-3和CleavedPARP蛋白表达量显著低于损伤组(p<0.05),随着F2浓度的升高,Cleaved Caspase-3表达量分别下降至280.90%、232.46%、185.26%,Cleaved PARP表达量随着F2浓度升高而降低至110.36%、85.85%、61.95%;F2高浓度组的Bcl-2/Bax比值(73.94%)较损伤组比显著提升(p<0.05)。表明人参皂苷F2可以抑制凋亡蛋白Caspase的活性,降低Bax促凋亡蛋白与Caspase家族上、中、下游蛋白的表达,通过调控Caspase级联反应抑制H2O2刺激引起的细胞凋亡。In this study, the anti-apoptotic effect of ginsenoside F2 on H2O2-induced injury in human embryonic kidney HEK-293 cells was evaluated. Using commercial kits, the activities of Caspase-6 and Caspase-9 of apoptotic cells were examined by measured. Western blot tests were conducted to detect the protein expression of the Bcl-2 family(Bcl-2 and Bax) and Caspase family(Cleaved Caspase-3 and Cleaved PARP). After the cells were injured by H2O2, the activity of Caspase increased and the expressions of pro-apoptotic proteins increased. The activities of Caspase-6 and Caspase-9 decreased in a concentration-dependent manner(p<0.05) after the pretreatment of cells with 1.25, 5, 20 μmol/L ginsenoside F2 to injure cells(p<0.05). At the F2 concentration of 20 μmol/L, the activities of Caspase-6 and Caspase-9 decreased by 6.83 U/mg protein and 5.88 U/mg protein., respectively, and the protein expression levels of Cleaved Caspase-3 and Cleaved PARP were significantly lower than those of the injured group(p<0.05). With the increase of F2 concentration, the expression of Cleaved Caspase 3 dropped to 280.90%, 232.46% and 185.26%, respectively. The expressions of Cleaved PARP decreased to 110.36%, 85.85% and 61.95%, respectively, with the increase of F2 concentration. The ratio of Bcl-2/Bax for the high F2 concentration group(73.94%) was significantly higher than that of the injured group(p<0.05). These results showed that ginsenoside F2 can inhibit the activity of apoptotic protein Caspase, and reduce the expression of Bax pro-apoptotic protein and the upstream, midstream and downstream proteins of the Caspase family, through inhibiting H2O2-induced apoptosis via regulating the Caspase cascade reactions.

关 键 词：人参皂苷 抗凋亡 HEK-293细胞 人参 

分 类 号：R285.5[医药卫生—中药学]