蚯蚓提取液对家蚕生长发育及血淋巴氧化指标的影响  

Effects of earthworm extract on growth and development of Bombyx mori and oxidation index of hemolymph

作　　者：吴永胜[1] 徐麒麟 杨家棣 罗红琼 杨雪[1] 邱时秀[1] 孙越鸿[1] 曹雨辰[1] 齐桂兰[1] WU Yongsheng;XU Qilin;YANG Jiadi;LUO Hongqiong;YANG Xue;QIU Shixiu;SUN Yuehong;CAO Yuchen;QI Guilan(Institute of Animal Science,Chengdu Academy of Agriculture and Forestry Sciences,Chengdu,Sichuan Province 611130,China;College of Animal Science and Technology,Sichuan Agricultural University,Chengdu,Sichuan Province 611130,China;Sericultural Bureau of Lezhi,Lezhi,Sichuan Province 641500,China)

机构地区：[1]成都市农林科学院畜牧研究所,四川成都611130 [2]四川农业大学动物科技学院,四川成都611130 [3]乐至县蚕桑局,四川乐至641500

出　　处：《中国饲料》2020年第15期18-21,共4页China Feed

基　　金：成都市2018年科研创新和集成应用示范(020204923);成都市2019年科研创新和集成应用示范(020207111)。

摘　　要：为探究蚯蚓提取液对家蚕生长发育及血淋巴氧化指标的影响,本试验以5龄家蚕幼虫为试验对象,分别饲喂不同稀释比例(50倍、100倍、200倍)的蚯蚓提取液处理的桑叶。结果显示:3个稀释组家蚕体质量均显著高于对照组(P<0.05),50倍稀释组家蚕存活率显著高于对照组(P<0.05);各稀释组家蚕全茧量、茧层量和茧层率差异不显著(P>0.05),但粒茧丝长、解舒率、解舒丝长、出丝率、洁净等丝质指标均有提高;与对照组相比,100和200倍稀释组家蚕血淋巴中丙二醛(MDA)含量分别降低38.04%和32.23%(P<0.05),乳酸脱氢酶(LDH)含量分别降低了25.65%和35.60%(P<0.05),各处理组总抗氧化能力(T-AOC)差异不显著(P>0.05)。根据试验结果初步认为,桑叶中添食蚯蚓提取液可促进家蚕生长发育,提高蚕丝品质,降低血淋巴中丙二醛和乳酸脱氢酶水平,本试验结果为蚯蚓提取液在生产上的应用提供参考。In order to study the effects of earthworm extract on growth and development of Bombyx mori and oxidation index of hemolymph.The 5 th instar larvae of Bombyx mori were used as the experimental object.They were fed with different dilution ratios(50,100,200 fold dilution)of mulberry leaves.Results showed that the body weight of silkworms in three dilution groups were significantly higher than the control group(P<0.05).The survival rate of silkworms in the 50-fold dilution group was significantly higher than the control group(P<0.05).The cocoon weight,cocoon shell weight and cocoon shell rate in each groups had no significant diffenence(P>0.05),but the cocoon filament length,reelability,length of non-broken cocoon filament,raw silk rate and neatness were all improved.Compared with the control group,the malondialdehyde(MDA)contents in the hemolymph of the 100 and 200-fold dilution groups were decreased by 38.04%and32.23%,respectively(P<0.05),the level of lactate dehydrogenase(LDH)respectively decreased by 25.65%and 35.60%(P<0.05).While the total antioxidant capacity(T-AOC)in each groups had no significant diffenence(P>0.05).It was concluded that adding the earthworm extract could promote the growth and development of silkworm,improve the quality of silk and reduce the levels of MDA and LDH in hemolymph.These results may provide a reference for the application of earthworm extract in production.

关 键 词：蚯蚓提取液 生长发育 氧化指标 血淋巴 家蚕 

分 类 号：S88[农业科学—特种经济动物饲养]