上海地区50例哮喘儿童厚苔及腻苔与舌面菌群的相关性研究  

Research on the relationship between thick fur,greasy fur and tongue microflora in 50 asthmatic children in Shanghai

作　　者：陈佳[1] 鲁晓篝 宋雪阳 孙克兴 王忆勤 CHEN Jia;LU Xiao-gou;SONG Xue-yang;SUN Ke-xing;WANG Yi-qin(Shanghai Children's Medical Center Affiliated to Shanghai Jiao Tong University School of Medicine,Shanghai 200127,China;Shanghai University of Traditional Chinese Medicine,Shanghai Key Laboratory of Health Identification and Assessment Laboratory/Laboratory of Traditional Chinese Medicine Four Diagnositic Information,Shanghai 201203,China)

机构地区：[1]上海交通大学医学院附属上海儿童医学中心,上海200127 [2]上海中医药大学,上海市健康辨识与评估重点实验室/中医四诊信息化实验室,上海201203

出　　处：《中华中医药杂志》2020年第7期3346-3349,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金面上项目(No.81673880);中国博士后科学基金第61批面上项目(No.2017M611608);上海市进一步加快中医药事业发展三年行动计划“杏林新星”项目[No.ZY(2018-2020)-RCPY-3014];上海交通大学“交大之星”计划医工交叉研究基金(No.YG2019QNA06)。

摘　　要：目的:探究哮喘儿童厚苔、腻苔和舌面菌群的关系。方法:运用中医舌面诊一体仪记录50例哮喘儿童的舌象参数,同时获取舌苔标本。根据舌苔厚薄指数和腐腻指数,筛选出厚苔26例,薄苔19例,腻苔13例,非腻苔37例样本,进行DNA抽提和PCR扩增,采用高通量测序技术对样本中的16S rRNA基因进行测序分析。结果:50个舌苔样本中厚苔组鉴定出菌属181个,薄苔组167个;腻苔138个,非腻苔组217个。仅在哮喘儿童厚苔组发现的菌属主要有拟杆菌、根瘤菌、普雷沃氏菌、粗杆菌、黄杆菌、粪球菌、噬甲基菌、厌氧绳菌、Sphaerochaeta、支原体等。其中,普氏菌-7属厚苔组较薄苔组丰度显著增加(P<0.05)。仅在哮喘儿童腻苔组发现的菌属主要有JG30-KF-AS9、Faecalibaculum、肠球菌、小红卵菌、Erysipelotrichaceae、变形菌、Ruminiclostridium_6、Falsirhodobacter。孪生球菌属丰度在腻苔组较非腻苔组显著增加(P<0.05)。结论:不同的菌群结构可能是形成哮喘儿童不同舌苔特征的原因之一。Objective:To explore the relationship between thick fur,greasy fur and tongue microflora in asthmatic children.Methods:Using TCM tongue diagnosis instrument,the tongue parameters of 50 children with asthma were recorded and the tongue coating specimens were obtained.According to the coating thickness index and coating decay index,26 cases of thick coating,19 cases of thin coating,13 cases of greasy coating and 37 cases of non-greasy coating were screened out.DNA extraction and PCR amplification were performed on all samples,and high-throughput sequencing technology of 16 s rRNA analysis was conducted.Results:From 50 tongue coating samples,181 genera of bacteria were identified in the thick fur group,167 in the thin fur group.138 in the greasy fur group and 217 in the non-greasy fur group.Bacteroides,rhizobia,prevotella,bacillus crude,flavobacter,coprococcus faecalis,methophage,anaerobic rope bacteria,Sphaerochaeta and mycoplasma were only found in the thick fur group.Among them,the relative abundance of prucella-7 in the thick fur group was significantly increased compared with that in the thin fur group(P<0.05).JG30-KF-AS9,Faecalibaculum,enterococcus,microoocyte,Erysipelotrichaceae,proteobacteria,Ruminiclostridium 6 and Falsirhodobacter were only found in the greasy fur group.The relative abundance of Gemella Berger in the greasy fur group was significantly increased compared with that in the non-greasy fur group(P<0.05).Conclusion:Different microflora structure may be one of the reasons for different tongue coating characteristics in children with asthma.

关 键 词：儿童哮喘 舌诊 菌群 高通量测序 

分 类 号：R272[医药卫生—中医儿科学]