电针印堂与大肠俞穴对肠易激综合征模型大鼠结肠动力的影响  

Effects of electroacupuncture at Yintang(EX-HN3)and Dachangshu(BL25)on colonic motility in irritable bowel syndrome rats

作　　者：王一凡 郭孟玮[1] 覃颖 朱文莲[1] 蓝莹[1] 王珊 吉毛先 张红林[1] 任晓暄[1] WANG Yi-fan;GUO Meng-wei;QIN Ying;ZHU Wen-lian;LAN Ying;WANG Shan;JI Mao-xian;ZHANG Hong-lin;REN Xiao-xuan(School of Acupuncture-Moxibustion and Tuina,Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学针灸推拿学院,北京100029

出　　处：《中华中医药杂志》2020年第7期3379-3382,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81473776);北京中医药大学校级科研纵向发展基金(No.2019-ZXFZJJ-035)。

摘　　要：目的:观察比较电针印堂与大肠俞穴对肠易激综合征(IBS)大鼠结肠动力的影响,探讨其相关机制。方法:40只Wistar新生大鼠随机分为空白组、模型组、印堂组和大肠俞组。应用母子分离、醋酸灌肠和结直肠扩张法联合制备IBS大鼠模型。印堂组和大肠俞组分别予电针相应穴位干预,20min/次,隔日1次,共5次。记录Bristol粪便评分,腹部回撤反射(AWR)的潜伏期、90s内收缩波个数、峰峰值,免疫组化法检测迷走神经背核(DMV)和结肠肌层多巴胺D2受体(D2R)的表达。结果:与空白组比较,模型组Bristol评分显著升高、AWR的潜伏期显著缩短、收缩波个数显著增多、峰峰值显著上升、DMV和结肠肌层D2R阳性表达显著降低(P<0.01);与模型组比较,印堂组、大肠俞组的Bristol评分均显著下降(P<0.05,P<0.01),DMV及结肠肌层中D2R阳性表达显著升高(P<0.01,P<0.05);大肠俞组潜伏期显著上升,收缩波个数和峰峰值均显著降低(P<0.01),且大肠俞组优于印堂穴组(P<0.01,P<0.05)。结论:电针印堂与大肠俞穴均可缓解IBS模型大鼠腹泻症状,但电针大肠俞穴作用更强,证实了经穴效应特异性存在且具有相对性。Objective:To observe and compare the effects of electroacupuncture(EA)at Yintang(EX-HN3)and Dachangshu(BL25)for colonic motility in irritable bowel syndrome(IBS)rats,and to explore its relevant meridians.Methods:Forty Wistar newborn rats were randomly and evenly divided into blank,model,EX-HN3 and BL25 groups.Combining motherchild separation,acetic acid enema and colorectal distension to establish IBS model.At the age of 9 weeks,EA at EX-HN3 and BL25 for 20 minutes were applied in the corresponding groups once every other day for 5 times.Record the Bristol stool score and 3 index of abdominal retraction reflex(AWR):the latent period before the first contraction wave,the number of contraction waves in 90s,and the peak-peak value.The expression of dopamine D2 receptor(D2R)was detected by immunehistochemistry.Results:Compared with the blank group,the Bristol stool scores of model group increased,the latent period was shorter,the peak-peak value and the number of contraction waves increased,the immunoreactive expression of D2R in DMV and the colon’s muscular layer decreased(P<0.01).Compared with the model group,the Bristol stool scores of EX-HN3 and BL25 groups decreased(P<0.05,P<0.01),the D2R in DMV and colon’s muscular layer increased(P<0.01,P<0.05);the latent period of BL25 group was longer,the number of contraction waves and the peak-peak value decreased(P<0.01),which was better than those of EX-HN3 group(P<0.01,P<0.05).Conclusion:EA at EX-HN3 and BL25 can both relieve diarrhea in IBS rats,but BL25 is better,which indicates the existence of therapeutic specificity and relativity of acupoints:Acupoints on different meridians can be used to treat the same disease yet with different effects.

关 键 词：肠易激综合征 印堂 大肠俞 多巴胺D2受体 结肠动力 电针 

分 类 号：R245[医药卫生—针灸推拿学]