针刺对慢性不可预知应激小鼠焦虑行为及终纹床核PACAP的调节作用  

Effects of acupuncture on anxiety-like behavior of mice and PACAP of the bed nucleus of stria terminalis

作　　者：何建才[1,2] 吕鹏 张承舜[1] 魏大能[1] 唐佳[1] 余曙光 蔡定均[1] 肖夏[1] 周奇志[1] HE Jian-cai;LYU Peng;ZHANG Cheng-shun;WEI Da-neng;TANG Jia;YU Shu-guang;CAI Ding-jun;XIAO Xia;ZHOU Qi-zhi(School of Acupuncture-Moxibustion and Tuina,Chengdu University of TCM,Chengdu 610075,China;Wenjiang Outpatient Department,Chengdu University of TCM,Chengdu 610075,China)

机构地区：[1]成都中医药大学针灸推拿学院,成都610075 [2]成都中医药大学温江门诊部,成都610075

出　　处：《中华中医药杂志》2020年第7期3635-3638,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81173322);高等学校博士学科点专项科研基金博导类资助课题(No.20135132110009)。

摘　　要：目的:研究针刺对慢性不可预知应激小鼠焦虑行为与终纹床核(BNST)内垂体腺苷酸环化酶激活肽(PACAP)及其受体PAC1信号分子的影响,探讨针刺抗焦虑的PACAP中枢作用机制。方法:运用慢性不可预知应激(CUS)方法造模。选取神门、内关进行手针针刺30min/次,隔日1次,持续15d。运用高架十字迷宫检测小鼠焦虑水平,以ELISA法测定小鼠血浆皮质酮(CORT)的含量,利用免疫组化法检测小鼠BNST内PACAP、PAC1的表达。结果:与空白组比较,模型组OT%值显著降低(P<0.05),血浆CORT水平显著升高(P<0.01),BNST内PACAP表达显著减少(P<0.01);与模型组比较,针刺组OT%值、OE%值显著升高(P<0.05),血浆CORT水平显著降低(P<0.01),BNST内PACAP表达显著增加(P<0.01)。结论:针刺改善CUS小鼠焦虑行为可能与上调BNST内PACAP表达、降低HPA轴活性相关。Objective:To reveal the central pituitary adenylate cyclase activating polypeptide(PACAP)mechanism of acupuncture anxiolytic,the study invested the effect of acupuncture on PACAP/PAC1 in the bed nucleus of stria terminalis(BNST)of mice.Methods:Anxious mouse model of CUS was established based on the literature.The acupuncture group selected Shenmen(HT7)and Neiguan(PC6)for acupuncture treatment for 30 minutes,followed by twirling of needle every 5 minutes,30 seconds for each time,treatment continued for 15 days.Elevated plus maze test(EPM)was used to test the anxiety-like behaviors for each group.The plasma corticosterone(CORT)level was determined by ELISA.Immunohistochemistry was used to detect the expression levels of PACAP,PAC1 in BNST.Results:Compared with the blank group,OT% in the model group decreased significantly(P<0.05),plasma CORT levels increased significantly(P<0.01),the expression of PACAP in the BNST reduced significantly(P<0.01);Compared with the model group,OT% and OE% in the acupuncture group increased significantly(P<0.05),the plasma CORT levels decreased significantly(P<0.01),the expression of PACAP in the BNST increased significantly(P<0.01).Conclusion:Anti-anxiety effects of acupuncture may be related with the suppression activation of the HPA axis and the upregulation expression of PACAP in the BNST.

关 键 词：针刺 焦虑障碍 终纹床核 垂体腺苷酸环化酶激活肽 PAC1 皮质酮 

分 类 号：R245[医药卫生—针灸推拿学]