3种药敏检测方法对洋葱伯克霍尔德菌体外抗菌药物药敏结果分析  

Study on the antimicrobial susceptibility of Burkholderia cepacia in vitro by three methods

作　　者：李莹[1] 陶运娟 徐杰[2] 任亚璐[2] 陈旭[2] LI Ying;TAO Yunjuan;XU Jie;REN Yalu;CHEN Xu(Department of Clinical Laboratory,Yancheng Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Traditional Chinese Medicine,Yancheng,Jiangsu 224000,China;Department of Clinical Laboratory,the First Affiliated Hospital of Suzhou University,Suzhou,Jiangsu 215000,China)

机构地区：[1]南京中医药大学附属盐城市中医院检验科,江苏盐城224000 [2]苏州大学附属第一医院检验科,江苏苏州215000

出　　处：《检验医学与临床》2020年第15期2160-2163,共4页Laboratory Medicine and Clinic

摘　　要：目的评估微量肉汤稀释法、纸片扩散法和E-test法检测洋葱伯克霍尔德菌体外抗菌药物药敏试验的结果。方法采用微量肉汤稀释法、纸片扩散法和E-test法检测31株洋葱伯克霍尔德菌对复方磺胺甲噁唑、头孢他啶、头孢哌酮/舒巴坦、米诺环素、左氧氟沙星和美罗培南这6种抗菌药物的药敏性。以微量肉汤稀释法为参考方法,计算50%最低抑菌浓度(MIC 50)、敏感率和误差率,分析基本一致率和分类一致率。结果复方磺胺甲噁唑的MIC 50为1.00μg/mL,敏感率为100.0%,体外活性最好。头孢哌酮/舒巴坦的MIC 50为16.00μg/mL,敏感率只有61.3%,体外活性最差。以微量肉汤稀释法为参考方法,E-test法检测头孢他啶、头孢哌酮/舒巴坦和左氧氟沙星的基本一致率和分类一致率均<90.0%,且存在一定的误差率,其中左氧氟沙星的非常重大误差为6.5%,头孢哌酮/舒巴的重大误差为12.9%;纸片扩散法检测头孢他啶和头孢哌酮/舒巴坦的分类一致率<90.0%,且存在一定的误差率,其中米诺环素的非常重大误差为6.5%,头孢哌酮/舒巴坦的重大误差为12.9%;E-test法和纸片扩散法检测复方磺胺甲噁唑和美罗培南敏感率均>90.0%,分类一致率均>90.0%。结论微量肉汤稀释法、纸片扩散法和E-test法检测洋葱伯克霍尔德菌可能存在不同的误差,应合理选择药敏检测方法,以确保结果的准确性。Objective To evaluate the results of antimicrobial susceptibility test of Burkholderia cepacia in vitro by micro-broth dilution,paper diffusion and E-test.Methods The susceptibility of 31 Burkholderia cepacia strains to compound Sulfamethoxazole,Ceftazidime,Cefoperazone/Sulbactam,Minocycline,Levofloxacin and Meropenem were tested by micro-broth dilution,paper diffusion and E-test.The 50%minimal inhibitory concentration(MIC 50),sensitivity rate and error rate were calculated,and the basic consistency rate and classification consistency rate were analyzed.Results The MIC 50 of compound Sulfamethoxazole was 1.00μg/mL,the sensitivity rate reached 100.0%,and the activity in vitro was the best.The MIC 50 of Cefoperazone/Sulbactam was 16.00μg/mL,the sensitivity rate was only 61.3%,and the activity in vitro was the worst.With the method of micro-broth dilution as reference,the basic consistency rate and classification consistency rate of Ceftazidime,Cefoperazone/Sulbactam and Levofloxacin were less than 90.0%by E-test,and there was a certain error rate,the very significant error of Levofloxacin was 6.5%,and the significant error of Cefoperazone/Sulbac was 12.9%,respectively.The classification consistency rate of Cefotaxime and Cefoperazone/Sulbactam was less than 90.0%by paper diffusion method,and there was a certain error rate.The very significant error of Minocycline was 6.5%,and that the significant error of Cefoperazone/Sulbactam was 12.9%.The sensitive rate of E-test and paper diffusion method to compound Sulfamethoxazole and Meropenem was more than 90.0%,the classification consistent rate was more than 90.0%.Conclusion There may be different errors in the detection of Burkholderia cepacia by the methods of micro-broth dilution,paper diffusion and E-test.The drug sensitivity detection method should be selected reasonably to ensure the accuracy of the results.

关 键 词：洋葱伯克霍尔德菌 E-TEST法 纸片扩散法 药敏试验 

分 类 号：R446.5[医药卫生—诊断学]