宁夏枸杞对枸杞瘿螨为害的内源激素响应及外源水杨酸对枸杞瘿螨的影响  

Hormone response of Lycium barbarum to gall mite Aceria pallida and effects of exogenous salicylic acid on the infestation and development of gall mite

作　　者：杨孟可 李建领[1,2] 刘赛 乔海莉[1] 郭昆[1] 徐荣[1] 陈君[1] 徐常青[1] YANG Meng-ke;LI Jian-ling;LIU Sai;QIAO Hai-li;GUO Kun;XU Rong;CHEN Jun;XU Chang-qing(Institu te of Medicinal Plant Development,Chinese Academy of Medical Science and Peking Union Medical College,Beijing 100193,China;China Resources Sanjiu Medical&Pharmaceutical Co.,Ltd.,Shenzhen 518110,Guangdong,China)

机构地区：[1]中国医学科学院-北京协和医学院药用植物研究所,北京100193 [2]华润三九医药股份有限公司,广东深圳518110

出　　处：《应用生态学报》2020年第7期2307-2313,共7页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金项目(81673699,81274198);中国医学科学院医学与健康科技创新工程项目(CIFMS 2016-I2M-3-017);国家重点研发计划项目(2017YFC1700706)资助。

摘　　要：为探索宁夏枸杞对枸杞瘿螨致瘿过程诱导的激素响应及外源水杨酸对枸杞瘿螨的影响,利用LC-MS/MS技术对枸杞瘿螨为害后宁夏枸杞叶片中水杨酸(SA)、茉莉酸(JA)、生长素(Auxin)和脱落酸(ABA)4种内源激素进行测定,并通过浸叶法研究外源SA对枸杞瘿螨侵染过程、虫瘿生长和种群增长的影响。结果表明:枸杞瘿螨的致瘿行为可引发枸杞叶片中多种内源激素产生变化,其中SA和JA的变化最显著,分别是对照组含量的4.0和13.0倍,而Auxin和ABA的变化不显著;外源SA对枸杞瘿螨虫瘿生长有显著抑制作用,且随时间的延长,抑制作用增强,同时虫瘿内瘿螨种群数量受到显著抑制。枸杞瘿螨能够同时诱导枸杞产生SA和JA抗性,外源SA对枸杞瘿螨虫瘿生长和瘿螨种群数量均具有显著抑制作用,利用外源SA防治枸杞瘿螨具有一定的应用前景。To investigate the hormone responses of Lycium barbarum to Aceria pallida infestation and the effects of exogenous salicylic acid on the infestation and development of gall mite,four phytohormones(salicylic acid(SA),jasmonic acid(JA),Auxin,and abscisic acid(ABA))of L.barbarum leaves infested with A.pallida were determined by LC-MS/MS.Moreover,the effects of exogenous SA on the growth of gall and mite population were investigated.The results showed that contents of SA and JA in leaves infested with A.pallida were significantly higher(4.0 and 13.0 folds,respectively)than that in control,but that of Auxin and ABA did not change.The growth of gall was inhibited significantly by exogenous SA,with the inhibition increasing with the prolongation of treatment time.Meanwhile,mite population in galls treated with SA was significantly lower than that in control.Our results suggested that A.pallida could induce SA and JA resistance of L.barbarum,and that the exogenous SA could inhibit the growth of gall and mite population.Exogenous SA could be potentially used in A.pallida prevention.

关 键 词：植物防御 诱导抗性 植物激素 侵染 发育 

分 类 号：S435.671[农业科学—农业昆虫与害虫防治]