参附注射液调控大鼠心肌梗死模型microRNA-135a的表达机制  

Mechanism of Shenfu Injection Regulating the Expression of MicroRNA-135a in Rat Model of Myocardial Infarction

作　　者：苏天生[1] 罗继红[2] 卢静[1] 陈志斌[1] 王志民[1] 廖云海 林磊[1] SU Tiansheng;LUO Jihong;LU Jing;CHEN Zhibin;WANG Zhimin;LIAO Yunhai;LIN Lei(The Second People’s Hospital Affiliated to Fujian University of Traditional Chinese Medicine,Fuzhou 350003,China;不详)

机构地区：[1]福建中医药大学附属第二人民医院,福建福州350003 [2]福建中医药大学

出　　处：《中外医学研究》2020年第16期1-3,共3页CHINESE AND FOREIGN MEDICAL RESEARCH

基　　金：福建省教育厅中青年教师教育科研项目(JAT170260)。

摘　　要：目的:研究参附注射液对大鼠心肌梗死模型microRNA-135a的调控机制。方法:将60只健康SD大鼠随机分为三组,每组20只。模型组和参附注射液组行结扎冠状动脉左前降支(LAD)术建立心肌梗死模型,假手术组冠状动脉不做结扎。模型组和假手术组予以0.9%氯化钠注射液6.0 ml/(kg·d)腹腔注射,参附注射液组予以参附注射液6.0 ml/(kg·d)腹腔注射,连续给药12周。比较三组大鼠心肌质量指数。采用Realtime RT-PCR方法检测大鼠心肌microRNA-135a的表达。结果:参附注射液组和模型组大鼠全心质量指数及左心室重量指数均明显高于假手术组,且模型组高于参附注射液组,差异有统计学意义(P<0.01)。与假手术组比较,模型组大鼠心肌microRNA-135a表达量上调,差异有统计学意义(P<0.05);与模型组比较,参附注射液组大鼠心肌microRNA-135a表达量下调,差异有统计学意义(P<0.05)。结论:microRNA-135a在心肌梗死中可能发挥重要作用,参附注射液可能通过抑制microRNA-135a的上调,抑制心肌梗死大鼠心室重构,从而改善大鼠心功能。Objective:To explore the regulation mechanism of Shenfu Injection on microRNA-135a in rat model of myocardial infarction.Method:A total of 60 healthy SD rats were randomly divided into three groups,with 20 rats in each group.The left anterior descending coronary artery(LAD)was ligated in model group and Shenfu Injection group to establish myocardial infarction model,while the coronary artery in sham operation group was not ligated.Model group and sham operation group were given 0.9%Sodium Chloride Injection 6.0 ml/(kg·d)intraperitoneal injection,and Shenfu Injection group was given Shenfu Injection 6.0 ml/(kg·d)intraperitoneal injection for 12 weeks.The myocardial mass index of the three groups was compared.The expression of microRNA-135a in rat myocardial was detected by Realtime RT-PCR.Result:The whole-heart mass index and left ventricular mass index of rats in Shenfu Injection group and model group were significantly higher than those in sham operation group,and the model group was significantly higher than that in Shenfu Injection group,the differences were statistically significant(P<0.01).Compared with sham operation group,the expression level of microRNA-135a was up-regulated in the model group,the difference was statistically significant(P<0.05).Compared with the model group,the expression level of microRNA-135a in rat myocardial was down-regulated in Shenfu Injection group,and the difference was statistically significant(P<0.05).Conclusion:MicroRNA-135a may play an important role in myocardial infarction.Shenfu Injection may improve the cardiac function of rats by inhibiting the up regulation of microRNA-135a and inhibiting the ventricular remodeling of rats with myocardial infarction.

关 键 词：参附注射液 心肌梗死 microRNA-135a 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]