类风湿性关节炎患者血清中IL-35、VitD、IL-33及其受体ST2表达水平及临床意义  

Expression Levels of IL-35,VitD,IL-33 and Its Receptor ST2 in Serum of Rheumatoid Arthritis Patients and Their Clinical Significance

作　　者：郭燕妳 刘小君 许海涛 GUO Yanni;LIU Xiaojun;XU Haitao(Shenzhen Hospital(guangming),University of Chinese Academy of Sciences,Shenzhen 518106,China;不详)

机构地区：[1]中国科学院大学深圳医院(光明),广东深圳518106

出　　处：《中外医学研究》2020年第21期4-7,共4页CHINESE AND FOREIGN MEDICAL RESEARCH

基　　金：深圳市宝安区科技局立项项目(2019JD221)。

摘　　要：目的:了解类风湿性关节炎(rheumatoid arthritis,RA)患者血清中白细胞介素-35(IL-35)、25-羟基维生素D3[25-(OH)VitD3]、IL-33及其受体ST2(ST2)表达水平,并探讨其临床意义,为RA的诊断提供参考依据。方法:选择2017年3月-2019年4月来医院风湿科门诊或住院部就诊并确诊为RA患者129例为RA组,同期来医院体检的健康人群100名为HC组,采用酶联免疫吸附法(ELISA)检测血清中IL-35、IL-33及ST2水平,采用化学反光分析仪检测25-(OH)VitD3水平,并对检测结果进行统计分析。结果:RA组患者IL-35和25-(OH)VitD3水平分别为(23.80±10.97)pg/ml和(34.68±6.63)nmol/L,明显低于HC组的(91.54±22.52)pg/ml和(62.91±13.75)nmol/L,而IL-33和ST2水平分别为(29.56±3.83)pg/ml和(18.32±3.49)ng/ml,明显高于HC组的(6.37±0.91)pg/ml和(7.05±0.74)ng/ml,差异均有统计学意义(P<0.05);IL-35和25-(OH)VitD3水平随着RA患者病情严重程度的加重而降低,而IL-33和ST2水平随着RA患者病情严重程度的加重而升高,差异有统计学意义(P<0.001);RA男性患者25-(OH)VitD3水平为(45.79±8.36)nmol/L,明显高于女性患者的(31.01±6.27)nmol/L,差异有统计学意义(P<0.05),而IL-35、IL-33及ST2水平差异无统计学意义(P>0.05);经Pearson相关分析,IL-35与25-(OH)VitD3水平呈明显正相关(P<0.05),与IL-33和ST2水平呈明显负相关(P<0.05),而IL-33与ST2水平呈明显正相关(P<0.05)。结论:RA患者IL-35和25-(OH)VitD3水平明显降低,而IL-33和ST2水平明显升高,且与病情密切相关。因此,加强IL-35、25-(OH)VitD3、IL-33及ST2水平监测,对RA患者的诊断及病情判断具有一定的参考价值。Objective:To investigate the expression levels of interleukin-35(IL-35),25-hydroxyvitamin D3[25-(OH)VitD3],IL-33 and its receptor ST2(ST2)in serum of patients with rheumatoid arthritis(RA),and to explore its clinical significance,so as to provide reference for the diagnosis of RA.Method:From March 2017 to April 2019,129 patients who visited the outpatient department or inpatient department of the rheumatology department of the hospital and were diagnosed as RA patients were selected as RA group,at the same time 100 healthy people for physical examination were selected as HC group,by using enzyme-linked immunosorbent(ELISA)to detect the serum level of IL-35,IL-33 and ST2,using chemical reflective analyzer test 25-(OH)VitD3 level,and the testing results were analyzed.Result:The levels of IL-35 and 25-(OH)VitD3 in the RA group were(23.80±10.97)pg/ml and(34.68±6.63)nmol/L,respectively,which were significantly lower than(91.54±22.52)pg/ml and(62.91±13.75)nmol/L in the HC group,while the levels of IL-33 and ST2 were(29.56±3.83)pg/ml and(18.32±3.49)ng/ml,respectively,which were significantly higher than(6.37±0.91)pg/ml and(7.05±0.74)ng/ml in the HC group,the differences were statistically significant(P<0.05).The levels of IL-35 and 25-(OH)VitD3 decreased with the aggravation of disease severity in RA patients,while the levels of IL-33 and ST2 increased with the aggravation of disease severity in RA patients,the differences were statistically significant(P<0.001).The level of 25-(OH)VitD3 in male patients with RA was(45.79±8.36)nmol/L,significantly higher than(31.01±6.27)nmol/L in female patients,the differences were statistically significant(P<0.05),but the difference of IL-35,IL-33 and ST2 levels was not statistically significant(P>0.05).Pearson correlation analysis showed that IL-35 was positively correlated with 25-(OH)VitD3(P<0.05),negatively correlated with IL-33 and ST2(P<0.05),and positively correlated with il-33 and ST2(P<0.05).Conclusion:In patients with RA,the levels of IL-35 and 25-(OH)VitD3 were si

关 键 词：白细胞介素-35 25-羟基维生素D3 白细胞介素-33 可溶性生长刺激表达基因2 类风湿性关节炎 

分 类 号：R593.22[医药卫生—内科学]