水稻粒长QTL定位与主效基因的遗传分析  

Mapping Quantitative Trait Loci Associated with Grain Length and Genetic Analysis of Major Quantitative Loci in Rice

作　　者：郑跃滨 李智[1] 赵海燕[1] 朱光枫 廖芷依 竺正航 王兰[1] ZHENG Yuebin;LI Zhi;ZHAO Haiyan;ZHU Guangfeng;LIAO Zhiyi;ZHU Zhenghang;WANG Lan(Guangdong Provincial Key Laboratory of Plant Molecular Breeding, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou 510642, China)

机构地区：[1]华南农业大学广东省植物分子育种重点实验室,亚热带农业生物资源保护与利用国家重点实验室,广州510642

出　　处：《西北植物学报》2020年第4期598-604,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：广东省自然科学基金面上项目(2019A1515011826);广东省教育厅科技创新项目(2013KJCX0035)。

摘　　要：该研究利用短粒普通野生稻矮杆突变体和长粒栽培稻品种KJ01组配杂交组合F1,构建分离群体F2;并对该群体粒长进行性状遗传分析,利用平均分布于水稻的12条染色体上的132对多态分子标记对该群体进行QTL定位及主效QTLs遗传分析,为进一步克隆新的主效粒长基因奠定基础,并为水稻粒形育种提供理论依据。结果表明:(1)所构建的水稻杂交组合分离群体F2的粒长性状为多基因控制的数量性状。(2)对543株F2分离群体进行QTL连锁分析,构建了控制水稻粒长的连锁遗传图谱,总长为1713.94 cM,共检测出24个QTLs,只有3个表现为加性遗传效应,其余位点均表现为遗传负效应。(3)检测到的3个主效QTLs分别位于3号染色体的分子标记PSM379~RID24455、RID24455~RM15689和RM571~RM16238之间,且三者对表型的贡献率分别为54.85%、31.02%和7.62%。(4)在标记PSM379~RID24455之间已克隆到的粒长基因为该研究新发现的主效QTL位点。In this study,we constructed an F2 isolated population derived from a cross between a dwarf wild rice mutant with short-grain and a cultivar variety KJ01 with long-grain.And the genetic analysis for grain length of the F2 population was performed.By using 132 pairs of polymorphic molecular markers evenly distributed on 12 chromosomes of rice,we conducted QTL mapping and major QTLs analysis,which would establish a foundation of further cloning the novel major grain length gene and provide a theoretical basis for grain shape breeding of rice.The results were as follows:(1)grain length trait of the isolated population F2 was quantitative trait controlled by multiple genes.(2)By QTL linkage analysis of 543 F2 individuals,a linkage map of 1713.94 cM controlling rice grain length was constructed,and a total of 24 QTLs were detected,and only 3 QTLs showed additive genetic effects,the others showed negative genetic effects.(3)The three major QTLs located in interval markers of PSM379-RID24455,RID24455-RM15689,and RM571-RM16238 on chromosome 3,which explained phenotypic variation for 54.85%,31.02%,and 7.62%,respectively.(4)The grain length QTL located in interval markers of PSM379-RID24455 was the major QTL newly discovered in this study.

关 键 词：水稻 粒长 QTL 遗传分析 

分 类 号：Q348[生物学—遗传学] Q343.17