头孢霉素对拟南芥主根生长发育的影响  

Effect of Cefotaxime on the Primary Root Growth and Development in Arabidopsis thaliana

作　　者：向敏 杨玲平 陈玉莲 秦甜甜 南文斌 XIANG Min;YANG Lingping;CHEN Yulian;QIN Tiantian;NAN Wenbin(Chongqing Key Laboratory of Molecular Biology of Plants Environments, College of Life Sciences, Chongqing Normal University, Chongqing 401331, China)

机构地区：[1]重庆师范大学生命科学学院,重庆市植物环境适应分子生物学重庆重点实验室,重庆401331

出　　处：《西北植物学报》2020年第4期605-612,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(31501190);重庆市基础研究与前沿探索项目(cstc2018jcyjA1615);重庆市教委科学技术项目(KJ1600303)。

摘　　要：以拟南芥野生型和相关转基因株系为材料,设置0、50、100、200和400μg/mL头孢霉素处理,考察头孢霉素对主根伸长生长、根尖分生组织活性、生长素分布运输以及干细胞活性的影响,探究头孢霉素对拟南芥主根生长发育的毒性作用机制。结果显示:(1)头孢霉素能以浓度依赖的方式抑制拟南芥主根的生长,并抑制分生组织长度和CYCB1;1基因的表达,说明它能抑制根尖分生组织活性。(2)头孢霉素能降低根尖生长素报告基因DR5∷GUS、DR5∷GFP和生长素极性运输蛋白PIN1、PIN2、PIN3、PIN7和AUX1的表达,说明它能抑制根尖生长素的分布和极性运输。(3)头孢霉素能下调根尖静止中心标记系WOX5∷GFP、QC25和QC46的表达,以及SHR和SCR蛋白的表达,说明它能抑制根尖干细胞活性。研究表明,头孢霉素能通过抑制根尖分生组织活性、生长素的分布和极性运输以及干细胞活性,从而调节拟南芥主根的生长发育。In this study,we subjected Arabidopsis thaliana wild type and related transgenic lines to the concentration of 0,50,100,200 and 400μg/mL cefotaxime,investigated the effects of cefotaxime on the elongation of primary root,meristem activities,auxin distribution and transport and stem cell activities,explored the toxic mechanism of cefotaxime on the growth and development of primary root.The results showed that:(1)cefotaxime remarkably inhibited primary root growth of A.thaliana in a concentration-dependent manner.It also repressed the meristem size and the expression of CYCB1;1,suggesting that cefotaxime can inhibit the meristem activities of root tips.(2)Cefotaxime reduced the expression of auxin reporter genes DR5∷GUS,DR5∷GFP and auxin polar transport proteins PIN1,PIN2,PIN3,PIN7 and AUX1,indicating that it can inhibit the auxin distribution and polar transport of root tips.(3)Cefotaxime down-regulated the expression of quiescent center marker lines WOX5∷GFP,QC25 and QC46,as well as the expression of SHR and SCR proteins,indicating that it can inhibit the stem cell activity of root tips.Collectively,cefotaxime regulates the growth and development primary root in A.thaliana by repressing the auxin distribution and transport,stem cell activity and meristem activity.

关 键 词：头孢霉素 拟南芥 生长素 主根 

分 类 号：Q256[生物学—细胞生物学] Q789