纳帕海高原湿地mazG遗传多样性初步研究  

Preliminary Study on Genetic Diversity of mazG in the Napahai Plateau Wetland

作　　者：崔自红 李珊[1] 秦堃豪 张琦[1] 魏云林[1] 唐兵 季秀玲[1] Cui Zihong;Li Shan;Qin Kunhao;Zhang Qi;Wei Yunlin;Tang Bing;Ji Xiuling(Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming,650500;The State Key Laboratory of Virology,Wuhan University,Wuhan,430072)

机构地区：[1]昆明理工大学,生命科学与技术学院,昆明650500 [2]武汉大学,病毒学国家重点实验室,武汉430072

出　　处：《基因组学与应用生物学》2020年第5期2148-2153,共6页Genomics and Applied Biology

基　　金：国家自然基金(31860147,31700324);云南省教育厅重点基金(2017ZZX132);病毒学国家重点实验室开放研究基金资助项目(2018KF003)共同资助。

摘　　要：mazG基因广泛存在于细菌和病毒中,其功能大多数未知,仅在大肠杆菌中的功能被证实。为揭示纳帕海高原湿地mazG遗传多样性,本研究于纳帕海高原湿地采集旱季泥炭土样品,提取总的基因组,以特异性引物对其mazG基因进行扩增,得到不同的mazG有效序列共39条,并与其它细菌及病毒进行系统发育分析和PCoA分析。通过研究得知,纳帕海高原湿地的部分mazG基因和其它细菌及病毒的mazG序列相接近,其余mazG序列的遗传距离较远,推测其可能为纳帕海高原湿地独有的类群。The mazG gene is widely distributed in bacteria and viruses.Its function is mostly unknown,and its function in E.coli is confirmed.In order to reveal the genetic diversity of mazG in the Napahai Plateau wetland,this study collected peat soil samples from the Napahai Plateau wetland in the dry season,extracted the total genome,and amplified the mazG gene with specific primers to obtain different effective mazG 39 sequences,and phylogenetic analysis and PCoA analysis with other bacteria and viruses.According to the research,some mazG genes in the Napahai Plateau wetland are similar to the maz G sequences of other bacteria and viruses,and the remaining mazG sequences have a long genetic distance.It is speculated that they may be unique to the Napahai Plateau wetland.

关 键 词：高原湿地 maz G基因 遗传多样性 Mothur软件 

分 类 号：Q943[生物学—植物学]