北部湾红树林沉积物中微生物群落结构的时空变化分析  

Spatiotemporal Analysis of Microbial Community Structure in the Mangrove Sediments in Beibu Gulf

作　　者：赵华显 阎冰[2] 徐悦[1] 莫淑名 聂世清 欧倩[1] 武波[1] 蒋承建[1,2] Zhao Huaxian;Yan Bing;Xu Yue;Mo Shuming;Nie Shiqing;Ou Qian;Wu Bo;Jiang Chengjian(State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources,College of Life Science and Technology,Guangxi University,Nanning,530004;Guangxi Key Laboratory of Mangrove Conservation and Utilization,Guangxi Mangrove Research Center,Guangxi Academy of Sciences,Beihai,536000)

机构地区：[1]广西大学亚热带农业生物资源保护与利用国家重点实验室,广西大学生命科学与技术学院,南宁530004 [2]广西红树林研究中心,广西科学院,北海536007

出　　处：《基因组学与应用生物学》2020年第5期2161-2169,共9页Genomics and Applied Biology

基　　金：国家重点研发计划科技基础资源调查专项(2017FY100704);广西红树林保护与利用重点实验室开放基金(课题编号:GKLMC-201702);广西“红树林和海草系统保育与生态监测”特聘专家岗基金资助。

摘　　要：微生物群落结构对海洋红树林湿地生态系统中的物质循环非常关键。本研究从中国亚热带北部湾典型海洋红树林湿地生态系统中采集沉积物样品,利用宏基因组学技术手段,完成了沉积物样品16S rDNA基因的扩增子的高通量测序,分析了不同时空条件下红树林沉积物的微生物群落结构分布和变化规律。研究结果表明,不管在干季还是湿季,在门分类水平上变形菌门(Proteobacteria)是最丰富的类别。此外,变形菌门(Proteobacteria)与绿弯菌门(Chloroflexi)、拟杆菌门(Bacteroidetes)、酸杆菌门(Acidobacteria)、硝化螺旋菌门(Nitrospirae)和放线菌门(Actinobacteria)一共占据总丰度的80%左右。干季时期的微生物α多样性显著高于湿季,主坐标分析(principal co-ordinates analysis,PCoA)显示干、湿两季的微生物群落结构具有明显差异。干季时期的细菌的相对丰度显著高于湿季,而古菌则相反。不同时空期(干季和湿季)的变形菌门(Proteobacteria)的相对丰度没有显著差异。冗余分析(redundancy analysis,RDA)和相关性分析表明温度和盐度对微生物群落结构具有显著影响。本研究对揭示亚热带海洋红树林湿地生态系统沉积物中的微生物多样性及其群落结构演替规律具有重要的实践意义。Microbial community structure plays a very important role in the matter cycling of marine mangrove wetland ecosystem.Samples were collected from the sediments in the typical subtropical marine mangrove wetland ecosystem in Beibu Gulf of China for the metagenomic analysis.A high-throughput sequencing of 16S rDNA gene amplicon was performed.The structure distribution and change rule of microbial community of mangrove sediments under different spatio-temporal conditions were analyzed.Proteobacteria is the most abundant phylum both in the dry and wet seasons.Proteobacteria,Chloroflexi,Bacteroidetes,Acidobacteria,Nitrospirae,and Actinobacteria account for about 80%of the total abundance.The microbial alpha diversity in the dry season is significantly higher than in the wet season.The principal coordinates analysis(PCoA)showed distinct differencein the microbial community structure between the two seasons.The relative abundance of bacterium in the dry season is significantly greater than in the wet season,whereas that of archaea showed the opposite trend.Under different spatio-temporal conditions(dry and wet seasons),no significant difference was found in the relative abun-dance of Proteobacteria.Redundancy analysis(RDA)and correlation analysis indicated that temperature and salinity have a significant impact on microbial community structure.This study has important practical significance for revealing microbial diversity and community structure succession rules in sediments of subtropical marine mangrove wetland ecosystems.

关 键 词：亚热带海洋红树林 时空变化 微生物群落结构 16S rDNA 

分 类 号：Q938.1[生物学—微生物学]