人参皂苷Rg1对APP/PS小鼠脑组织内Aβ沉积及行为学的影响  

The Effects of Ginsenoside Rg1 to the APP/PS1 Transgenic AD Model Mice on Behavioral Change and the Accumulation of Aβ in the Brain

作　　者：冯敏[1] 胡晶[2] 温洁馨 王言覃 徐佳妮 王治佳 Feng Min;Hu Jing;Wen Jiexin;Wang Yanqin;Xu Jia'ni;Wang Zhijia(Basic Medical College,Chongqing Medical University,Chongqing,400016;Laboratory Medical College,Chongqing Medical University,Chongqing,400016;School of Pediatrics,Chongqing Medical University,Chongqing,400016;First Clinical Medical College,Chongqing Medical University,Chongqing,400016)

机构地区：[1]重庆医科大学,基础医学院,重庆400016 [2]重庆医科大学,检验医学院,重庆400016 [3]重庆医科大学,儿科学院,重庆400016 [4]重庆医科大学,第一临床医学院,重庆400016

出　　处：《基因组学与应用生物学》2020年第5期2361-2366,共6页Genomics and Applied Biology

基　　金：重庆医科大学创新实验项目(No.201536)资助。

摘　　要：阿尔茨海默症(Alzheimer disease,AD)是一种常见神经退行性疾病,它的主要病理学特征为老年斑、基底前脑胆碱能神经元退变及神经纤维缠结。β淀粉样蛋白(amyloidβ-protein,Aβ)是老年斑的主要成分,过量的Aβ的产生和聚集与AD的发病有着密切的关系。而人参皂甙Rg1是人参促智的主要有效成分之一,对Aβ诱导的神经元的损害具有保护作用。本实验研究了人参皂苷Rg1对APP/PS转基因AD模型小鼠脑组织内APP代谢过程中相关蛋白的表达变化、小鼠行为学的影响。实验发现人参皂甙Rg1处理小鼠在Morris水迷宫实验中定位航行实验游泳路程逐日明显递减,且游泳路程较对照组明显缩短;皮质区老年斑数目较对照组显著减少(p<0.05),且Aβ沉积面积相比对照组更小;海马齿状回区与皮质区MAP-2着色阳性神经元较对照组明显增多(p<0.05),皮质区可见呈束状排列的神经纤维着色。结果提示人参皂苷Rg1能够显著改善AD模型小鼠的学习记忆能力,其作用机制可能与减少Aβ产生或抑制Aβ沉积、促进神经纤维的生长以及修复,从而减轻AD引起的脑组织受损程度有关。Alzheimer disease(AD)is one of the most common neurodegenerative diseases.The main pathological characteristics include senile plaques(SP),neural fibrillary tangles(NFTs)and the degeneration of cholinergic neurons in basal forebrain.Amyloidβ-protein(Aβ)is the major component of SP.It’s believed that the excessive production and accumulation of Aβclosely correlate with the pathogenesis of AD.Ginsenoside Rg1,one of the leading nootropic ingrediens in Ginseng,can prevent the neurons from the damage induced by Aβ.The main objective was to determine the effects of Ginsenoside Rg1 to the APP/PS1 transgenic AD model mice both on the expression of proteins relevant to metabolism of APP in the brain and the behaviors of the mice.We found that the swim distance of the mice treated with Ginsenoside Rg1 showed an apparent daily diminish,and manifest a notable short compared with the control group.For the SP,the quantity of it in the hippocampus and cortex area ofthe experimental group was fewer than the control group,especially in the cortex area(p<0.05).And the accumul-ation area tended to be smaller in the brain of the treated group as well.The MAP-2 staining neurons in the hippocampus and cortex area of the experimental group manifested a larger amount(p<0.05).Stained nerve fiber could be seen to array in bundle after ginsenoside Rg1 treatment,while that tangled in the control group.Taken together,ginsenoside Rg1 was capable to improve the learning and memorize ability of the AD model mice,with the possible mechanism correlated with reducing the neuron damage caused by AD.Our study indicated that it may have something to do with reducing the production of Aβor inhibit the accumulation of it,along with stimulating nerve fiber growth and repairing the damaged structure.

关 键 词：阿尔茨海默病 人参皂甙RG1 Β淀粉样蛋白 APP/PS1双转基因小鼠 

分 类 号：R285.5[医药卫生—中药学]