水稻NAM基因家族的全基因组鉴定及表达分析  

Genome-wide Identification and Expression Analysis of NAM Gene Family in Rice

作　　者：陈琼琼 Abdullah Shalmani 陈云波 黄洋彬 陈坤明[1] 李文强[1] CHEN Qiongqiong;SHALMANI Abdullah;CHEN Yunbo;HUANG Yangbin;CHEN Kunming;LI Wenqiang(State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China)

机构地区：[1]西北农林科技大学生命科学学院,旱区作物逆境生物学国家重点实验室,陕西杨陵712100

出　　处：《西北植物学报》2020年第6期907-917,共11页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(31570181);中央高校基本科研业务费专项基金(2452018149)。

摘　　要：植物特异性转录因子NAM家族从属于NAC转录因子超家族,在植株生长发育、生理代谢以及应对各种胁迫反应中均发挥重要作用。该研究采用生物信息学方法鉴定水稻基因组中的NAM基因,分析其时空表达模式、亚细胞定位以及蛋白相互作用,并采用实时定量qRT-PCR方法分析不同外源激素(如SA、ABA和MeJA)以及非生物胁迫(包括干旱、盐和冷)处理下各NAM基因的表达特征,为进一步探索NAM基因在非生物胁迫中的功能和应激机制以及激素调控途径奠定基础。结果显示:(1)从水稻基因组中共鉴定出48个NAM基因,进化分析将其分为5个亚家族;NAM基因在水稻基因组中存在9对片段复制事件。(2)组织表达分析显示,NAM基因在水稻不同组织及发育时期表现特异性表达,特别是叶鞘、茎和节的生长过程中高表达,且大多数是核定位,并存在多种蛋白互作。(3)实时定量qRT-PCR表达分析显示,10个NAM基因在不同组织中均特异表达;大部分NAM基因在盐和干旱胁迫下表达上调,而在冷胁迫下表达降低;SA、ABA和MeJA处理均可显著改变各NAM基因的表达水平。研究表明,NAM基因在水稻生长发育、激素应答和非生物胁迫响应中具有重要作用。Plant-specific transcription factors NAM family belongs to NAC transcription factor superfamily that play an important role in plant growth and development,physiological metabolism and response to various stresses.This study used bioinformatics methods to identify NAM genes in rice genome,analyzed their spatiotemporal expression patterns,subcellular localization and protein interaction,and used real-time quantitative qRT-PCR to analyze the expression characteristics of NAM genes under different exogenous hormones(such as SA,ABA and MeJA)and abiotic stresses(including drought,salt and cold),and laid the foundation for further exploring the function and stress mechanism of NAM genes in abiotic stress and hormone regulation pathways.The results showed:(1)48 NAM genes were identified from rice genome,clustered into five subfamilies through evolutionary analysis.There were nine pairs segmentally duplications of NAM genes in the rice genome.(2)Tissue expression analysis showed that NAM genes specifically expressed in different tissues and developmental stages of rice,especially high expression in the leaf sheath,stems and nodes;most of them are nuclear localization;there are many protein interactions.(3)Real-time quantitative qRT-PCR expression analysis showed that 10 NAM genes were specifically expressed in different tissues;most of NAM genes were upregulated under salt and drought stresses,while the expression was low under cold stress.;the transcript levels of NAM genes were highly altered under SA,ABA,and MeJA application.The results showed that NAM genes play an important role in rice growth and development,hormones response and abiotic stresses response.

关 键 词：水稻 NAM家族 非生物胁迫 激素应答 基因表达 

分 类 号：Q785[生物学—分子生物学] Q786