Syntaxin-1通过激活突触递质传递加速早期突触的形成  

Syntaxin-1 Accelerates Early Synapse Formation via Activating Synaptic Transmission

作　　者：裴青 林寅[1] 荣伊 刘孟雪 吴世平 阳小飞[1] PEI Qing;LIN Yin;RONG Yi;LIU Meng-Xue;WU Shi-Ping;YANG Xiao-Fei(Key Laboratory of Cognitive Science,Hubei Key Laboratory of Medical Information Analysis and Tumor Diagnosis&Treatment,Laboratory of Membrane Ion Channels and Medicine,College of Biomedical Engineering,South-Central University for Nationalities,Wuhan 430074,China)

机构地区：[1]认知科学重点实验室湖北省医学信息分析与肿瘤诊疗重点实验室膜离子通道与药物研发实验室中南民族大学生物医学工程学院,武汉430074

出　　处：《生物化学与生物物理进展》2020年第7期626-633,共8页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金(31670850);中南民族大学基础研究基金(CZY19022和CZY19040)资助项目~~。

摘　　要：Syntaxin-1是一种多结构域蛋白,通过与synaptobrevin-2和SNAP-25形成SNARE复合体调节囊泡融合.然而,syntaxin-1在突触形成过程中是否发挥作用,目前尚不清楚.本研究显示syntaxin-1的表达水平与突触形成过程高度相关.Syntaxin-1的R151A和I155A突变影响其在突触形成中的促进作用,而Habc结构域或跨膜结构域在突触形成中无显著作用.结果表明,syntaxin-1通过激活突触囊泡释放来加速突触的形成.yntaxin-1, known as a multi-domain protein, regulates vesicle fusion by forming SNARE complex with synaptobrevin-2 and SNAP-25. However, the role of syntaxin-1 in synapse formation remains uncovered.Here we demonstrated that the expression level of syntaxin-1 was highly associated with the process of synaptogenesis. The R151A and I155A mutations but not Habcor transmembrane domain truncations of syntaxin-1 impaired its facilitation in synapse formation. Our results suggested that syntaxin-1 accelerated synapse formation via activating synaptic vesicle release.

关 键 词：syntaxin-1 突触形成 突触囊泡 动作电位触发的胞吐作用 

分 类 号：Q189[生物学—神经生物学] R318[生物学—普通生物学]